Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V0I11_RS06065 Genome accession   NZ_CP143490
Coordinates   1291884..1293269 (-) Length   461 a.a.
NCBI ID   WP_330453240.1    Uniprot ID   -
Organism   Pasteurella multocida strain FCf147     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1286884..1298269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0I11_RS06035 (V0I11_06035) - 1287011..1288384 (-) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -
  V0I11_RS06040 (V0I11_06040) - 1288833..1289102 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  V0I11_RS06045 (V0I11_06045) yacG 1289102..1289305 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  V0I11_RS06050 (V0I11_06050) coaE 1289316..1289936 (-) 621 WP_005722564.1 dephospho-CoA kinase -
  V0I11_RS06055 (V0I11_06055) - 1289951..1290673 (-) 723 WP_170385408.1 prepilin peptidase -
  V0I11_RS06060 (V0I11_06060) - 1290673..1291884 (-) 1212 WP_250192716.1 type II secretion system F family protein -
  V0I11_RS06065 (V0I11_06065) pilB 1291884..1293269 (-) 1386 WP_330453240.1 GspE/PulE family protein Machinery gene
  V0I11_RS06070 (V0I11_06070) pilA 1293269..1293703 (-) 435 WP_061406215.1 prepilin peptidase-dependent pilin Machinery gene
  V0I11_RS06075 (V0I11_06075) ampD 1293863..1294429 (+) 567 WP_330453242.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V0I11_RS06080 (V0I11_06080) rppH 1295015..1295482 (+) 468 Protein_1164 RNA pyrophosphohydrolase -
  V0I11_RS06085 (V0I11_06085) - 1295614..1296408 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  V0I11_RS06090 (V0I11_06090) lgt 1296418..1297221 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  V0I11_RS06095 (V0I11_06095) - 1297227..1298078 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52144.81 Da        Isoelectric Point: 6.6310

>NTDB_id=929174 V0I11_RS06065 WP_330453240.1 1291884..1293269(-) (pilB) [Pasteurella multocida strain FCf147]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLNQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=929174 V0I11_RS06065 WP_330453240.1 1291884..1293269(-) (pilB) [Pasteurella multocida strain FCf147]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTAATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGTTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(127-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.433

98.265

0.672
  pilB Haemophilus influenzae Rd KW20

67.77

98.265

0.666
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

40.365

100

0.432
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.412
  pilB Acinetobacter baylyi ADP1

38.076

100

0.412
  pilB Vibrio cholerae strain A1552

38.477

100

0.406
  pilB Legionella pneumophila strain ERS1305867

37.423

100

0.397