Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KAN5_RS11350 Genome accession   NZ_AP025589
Coordinates   2545990..2547669 (+) Length   559 a.a.
NCBI ID   WP_244389729.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. KAN5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2540990..2552669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN5_RS11330 - 2541252..2542523 (+) 1272 WP_244389726.1 O-antigen ligase family protein -
  KAN5_RS11335 (KAN5_22160) pilC 2542537..2543766 (+) 1230 WP_244389727.1 type II secretion system F family protein Machinery gene
  KAN5_RS11340 (KAN5_22170) pilD 2544268..2545167 (+) 900 WP_279612612.1 A24 family peptidase Machinery gene
  KAN5_RS11345 (KAN5_22180) coaE 2545169..2545798 (+) 630 WP_244389728.1 dephospho-CoA kinase -
  KAN5_RS11350 (KAN5_22190) pilB 2545990..2547669 (+) 1680 WP_244389729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KAN5_RS11355 (KAN5_22200) yacG 2547730..2547960 (+) 231 WP_016708280.1 DNA gyrase inhibitor YacG -
  KAN5_RS11360 (KAN5_22210) - 2548287..2548475 (-) 189 WP_138553807.1 hypothetical protein -
  KAN5_RS11365 (KAN5_22220) - 2548476..2549708 (-) 1233 WP_244389730.1 divalent metal cation transporter -
  KAN5_RS11370 (KAN5_22230) raiA 2549843..2550199 (-) 357 WP_016708282.1 ribosome-associated translation inhibitor RaiA -
  KAN5_RS11375 (KAN5_22240) - 2550380..2550691 (-) 312 WP_244389731.1 pyrimidine/purine nucleoside phosphorylase -
  KAN5_RS11380 (KAN5_22250) - 2550900..2551367 (-) 468 WP_244389732.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62469.86 Da        Isoelectric Point: 5.4651

>NTDB_id=92883 KAN5_RS11350 WP_244389729.1 2545990..2547669(+) (pilB) [Pseudoalteromonas sp. KAN5]
MNINSPLLRKFITLGRIDAETVKLKQREYASTAELIAKCSGIDGHELAEQCIDLFRVPYFDLRDFDVELIPKELIKEKLI
KEHHILPLVKKGRKLYIAASDPTDYGAFENFEFSTGLSCEVVVVDYRQLEAKIELLLDASGSLHLSDEEFKEFSDLDTES
PHEPQNSNEDEKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRVRFRIDGILHEMASPPNSLATRLSARIKVMARLD
IAEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALQQPQGMILVTGP
TGSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPRAEMTFANALKAFLRQDPDVVMVGEIRDLETAEISI
KAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPETLPSEELLRQGFTPEQISELT
LFAPKGCDSCTEGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKSGFNNLRLSGLRKAADGLTSLAEINRVTSF

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=92883 KAN5_RS11350 WP_244389729.1 2545990..2547669(+) (pilB) [Pseudoalteromonas sp. KAN5]
ATGAATATTAATTCACCATTACTTAGAAAGTTTATTACGCTTGGTCGTATTGACGCAGAAACAGTCAAACTAAAACAACG
TGAGTATGCCTCAACAGCAGAGCTAATAGCAAAGTGCAGCGGTATTGATGGCCATGAACTTGCCGAGCAATGCATTGATT
TATTCCGAGTACCTTATTTTGATTTACGTGATTTTGATGTAGAGCTGATCCCTAAAGAGTTAATTAAAGAAAAGCTGATC
AAAGAACACCATATTCTGCCTTTGGTAAAAAAAGGCCGTAAGCTCTATATTGCAGCCTCTGATCCGACAGATTATGGTGC
CTTTGAGAATTTTGAATTCAGTACTGGGCTCTCTTGTGAAGTTGTCGTGGTCGATTATCGTCAACTCGAAGCAAAAATAG
AGCTGTTGCTTGATGCCTCAGGCAGCTTGCACTTAAGCGATGAAGAATTTAAAGAGTTTAGTGATCTCGATACTGAAAGC
CCACACGAGCCGCAAAACAGCAATGAAGATGAAAAAGACGATGCACCTATTATTGTCTACATTAATAAAATTTTAATGGA
TGCGATAAAAAAGGGCGCCTCCGATCTACACTTTGAACCCTATGAGCATAAATACCGCGTACGTTTTCGTATTGATGGTA
TTTTGCATGAAATGGCCAGCCCCCCTAACAGCCTTGCTACCCGCTTGTCAGCACGTATAAAAGTTATGGCGCGTTTAGAC
ATTGCCGAAAAACGTAAGCCGCAAGATGGGCGTATTAAATTAAAAATTACCGAACGTAAAAGCATTGATTTTCGGGTAAG
CACCCTGCCTACTTTATGGGGCGAGAAAATCGTAATGCGTATTCTTGACTCATCCAGTGCAATGCTAGGCATTGACGTAC
TTGGCTATGAACCTGAGCAGAAAAAGCTCTATATGGATGCGCTGCAACAACCACAAGGCATGATCTTAGTTACCGGCCCT
ACGGGCTCTGGTAAAACGGTTTCACTTTACACGGGATTGAATATTCTTAACCAACCAGAGCGTAATATTAGTACTGCGGA
AGATCCTGTTGAAATAAACCTCGAGGGGATCAACCAAGTACAAATTAACCCACGCGCCGAGATGACCTTTGCAAACGCGC
TAAAAGCCTTCTTACGTCAAGATCCTGATGTGGTAATGGTCGGTGAGATCCGTGATCTTGAAACTGCTGAAATATCTATT
AAAGCGGCACAAACAGGTCACTTAGTGCTATCAACACTGCATACTAACTCAGCACCTGAAACATTAACCCGTTTACTTAA
CATGGGGGTGCCTGCTTATAACGTGGCCAGTTCGATTAGTTTGATCATCGCGCAGCGTTTAGCACGACGTTTATGCCCTA
AATGTAAAACACCTGAAACACTGCCAAGTGAAGAGCTACTGCGCCAAGGCTTCACGCCTGAGCAAATAAGCGAACTGACT
TTATTTGCCCCTAAAGGTTGCGATAGCTGTACTGAAGGCTATAAAGGCCGTGTTGGTATTTATGAGGTAATGCAAATTAC
TCCAGAAATAGCGCAGATCATTATGCGTGGTGGCAACTCGTTAGAAATTGCAGAGGTATCGTTAAAGTCAGGTTTTAATA
ACTTGCGTTTATCGGGGCTTCGTAAAGCCGCAGATGGTTTAACTTCACTGGCCGAAATAAACCGCGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(183-450)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.895

91.95

0.56
  pilB Acinetobacter baylyi ADP1

61.1

91.055

0.556
  pilB Vibrio cholerae strain A1552

54.409

95.349

0.519
  pilB Legionella pneumophila strain ERS1305867

53.148

96.601

0.513
  pilB Vibrio campbellii strain DS40M4

52.239

95.886

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

53.269

93.023

0.496
  pilF Neisseria gonorrhoeae MS11

50.969

92.308

0.47

Multiple sequence alignment