Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACA342_RS25635 Genome accession   NZ_CP166817
Coordinates   5483808..5484347 (-) Length   179 a.a.
NCBI ID   WP_033938465.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain M7242404     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5478808..5489347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA342_RS25615 (ACA342_25615) - 5479493..5481748 (-) 2256 WP_033990664.1 DUF1631 domain-containing protein -
  ACA342_RS25620 (ACA342_25620) nadC 5482034..5482882 (+) 849 WP_033990663.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACA342_RS25630 (ACA342_25630) - 5483046..5483744 (-) 699 WP_223677410.1 hypothetical protein -
  ACA342_RS25635 (ACA342_25635) pilE 5483808..5484347 (-) 540 WP_033938465.1 pilin Machinery gene
  ACA342_RS25640 (ACA342_25640) pilB 5484576..5486279 (+) 1704 WP_371032314.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACA342_RS25645 (ACA342_25645) - 5486283..5486789 (+) 507 Protein_5074 type II secretion system F family protein -
  ACA342_RS25650 (ACA342_25650) - 5486792..5487994 (-) 1203 WP_003464938.1 ISL3 family transposase -
  ACA342_RS25655 (ACA342_25655) - 5488094..5488813 (+) 720 Protein_5076 type II secretion system F family protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18082.46 Da        Isoelectric Point: 6.2547

>NTDB_id=928682 ACA342_RS25635 WP_033938465.1 5483808..5484347(-) (pilE) [Pseudomonas aeruginosa strain M7242404]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVRIAEATGEITVTFNATNVGNIPADSTLVFTPYVQNAAGAPTQLGASYAAGVTGSIDWGCASQSNAVSSGPDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=928682 ACA342_RS25635 WP_033938465.1 5483808..5484347(-) (pilE) [Pseudomonas aeruginosa strain M7242404]
ATGAAAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGTCTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACGAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCACAAACGTGGGTAA
TATTCCGGCTGACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTGCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGCAATCCAATGCGGTTTCCAGTGGTCCCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCGAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.64

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

43.784

100

0.453
  pilA2 Legionella pneumophila str. Paris

41.808

98.883

0.413
  pilA2 Legionella pneumophila strain ERS1305867

41.243

98.883

0.408
  comP Acinetobacter baylyi ADP1

38.333

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

35.714

100

0.363