Detailed information NTG0928426

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: VT264_RS12450
• Genome accession: NZ_CP143266
• Coordinates: 2585754..2586368 (-)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain JBCS608
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2580754..2591368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VT264_RS12420 (VT264_12420)	rpsT	2581054..2581320 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
VT264_RS12425 (VT264_12425)	holA	2581337..2582378 (-)	1042	Protein_2401	DNA polymerase III subunit delta	-
VT264_RS12430 (VT264_12430)	-	2582418..2582572 (-)	155	Protein_2402	hypothetical protein	-
VT264_RS12435 (VT264_12435)	-	2582613..2582747 (+)	135	WP_003152870.1	YqzM family protein	-
VT264_RS12440 (VT264_12440)	comEC	2582766..2585117 (-)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VT264_RS12445 (VT264_12445)	-	2585118..2585687 (-)	570	WP_003152868.1	ComE operon protein 2	-
VT264_RS12450 (VT264_12450)	comEA	2585754..2586368 (-)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
VT264_RS12455 (VT264_12455)	comER	2586427..2587248 (+)	822	WP_014305449.1	late competence protein ComER	-
VT264_RS12460 (VT264_12460)	-	2587317..2588054 (-)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
VT264_RS12465 (VT264_12465)	rsfS	2588051..2588407 (-)	357	WP_003152864.1	ribosome silencing factor	-
VT264_RS12470 (VT264_12470)	yqeK	2588425..2588985 (-)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
VT264_RS12475 (VT264_12475)	-	2588975..2589544 (-)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
VT264_RS12480 (VT264_12480)	yhbY	2589555..2589845 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
VT264_RS12485 (VT264_12485)	aroE	2589839..2590681 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=928426 VT264_RS12450 WP_041482377.1 2585754..2586368(-) (comEA) [Bacillus velezensis strain JBCS608]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=928426 VT264_RS12450 WP_041482377.1 2585754..2586368(-) (comEA) [Bacillus velezensis strain JBCS608]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363