Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HQS1_RS05795 Genome accession   NZ_AP025556
Coordinates   1282456..1284198 (-) Length   580 a.a.
NCBI ID   WP_034364744.1    Uniprot ID   A0AAX3SVK0
Organism   Delftia lacustris strain HQS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1277456..1289198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQS1_RS05765 (HQS1_11400) mutT 1278038..1278544 (+) 507 WP_043789746.1 8-oxo-dGTP diphosphatase MutT -
  HQS1_RS05770 (HQS1_11410) - 1278555..1278791 (-) 237 WP_223275109.1 DNA gyrase inhibitor YacG -
  HQS1_RS05775 (HQS1_11420) zapD 1278809..1279564 (-) 756 WP_016445607.1 cell division protein ZapD -
  HQS1_RS05780 (HQS1_11430) coaE 1279654..1280292 (-) 639 WP_016451024.1 dephospho-CoA kinase -
  HQS1_RS05785 (HQS1_11440) pilD 1280307..1281200 (-) 894 WP_016445609.1 A24 family peptidase Machinery gene
  HQS1_RS05790 (HQS1_11450) pilC 1281200..1282417 (-) 1218 WP_016445610.1 type II secretion system F family protein Machinery gene
  HQS1_RS05795 (HQS1_11460) pilB 1282456..1284198 (-) 1743 WP_034364744.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HQS1_RS05805 (HQS1_11470) - 1284435..1285364 (-) 930 WP_034364039.1 polyprenyl synthetase family protein -
  HQS1_RS05810 (HQS1_11480) rplU 1285595..1285906 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  HQS1_RS05815 (HQS1_11490) rpmA 1285922..1286179 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  HQS1_RS05820 (HQS1_11500) cgtA 1286329..1287444 (+) 1116 WP_016451026.1 Obg family GTPase CgtA -
  HQS1_RS05825 (HQS1_11510) proB 1287528..1288676 (+) 1149 WP_016445651.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62761.81 Da        Isoelectric Point: 5.1481

>NTDB_id=92683 HQS1_RS05795 WP_034364744.1 1282456..1284198(-) (pilB) [Delftia lacustris strain HQS1]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAAGKAATNRTSFIAELSVAGTLSALEVAETVSTMFSAPLLDLD
AVDAMRLPQDLLDAKIISTYRVLPLSKRGNRLTVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQNKSASEA
MDSYGGSGDFEFGDLKPDEVAEEKDNSAAADVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPEE
KARLLEAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALIDAGYDDADIDGSWVTYRPVGCPACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=92683 HQS1_RS05795 WP_034364744.1 1282456..1284198(-) (pilB) [Delftia lacustris strain HQS1]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCATCCGCTGTCGCATCGCTTCCCGGCATCGGGCGGGCTCTGATTTCAGC
AGGCAAGGTATCGCAACAGGCAGCGGAAGCGGCTGCCGGCAAGGCCGCCACCAATCGCACCTCGTTCATTGCAGAACTGA
GCGTCGCCGGCACCCTCTCTGCCCTGGAAGTGGCGGAGACGGTTTCCACCATGTTCAGCGCTCCGCTGCTCGATCTTGAT
GCGGTCGACGCCATGCGCCTGCCGCAGGATCTGCTGGACGCCAAGATCATCAGCACCTATCGCGTCCTGCCTCTGAGCAA
GCGCGGCAACCGCCTCACGGTGGCCACCGCCGACCCGACGCAGCAGGAAGCCGCCGAGCAGATCAAGTTCACCACCCAGT
TGGGCGTGGACTGGATCATCGTCGAAGCCGACAAGCTGGAGCGACTGATCGCGCAGCAGAACAAAAGCGCCAGCGAGGCC
ATGGACAGCTATGGCGGCAGCGGCGACTTCGAGTTCGGCGACCTCAAGCCTGATGAGGTGGCAGAGGAAAAGGACAACTC
GGCCGCAGCAGACGTCGAAGATGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACTTGCATTTCGAACCCTACGAGCACCAGTACCGCGTGCGCTTTCGCATTGACGGCGAGCTGCGTGAAATTGCATCG
CCGCCCATTGCCATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGTGTCCC
GCAGGACGGGCGCATGAAGCTCAAGGTCGGTCCGGACCGCGTCATCGACTTTCGCGTCAGCACGCTGCCCACGCTTTTCG
GCGAGAAGATCGTGATCCGTATCCTGGACCCGAGCTCGGCCAGGATGGGCATCGACGCCCTGGGCTATGAACCCGAGGAG
AAGGCACGCCTGCTTGAAGCCATCAGCCGCCCCTACGGAATGATTCTGGTCACAGGCCCCACCGGCTCGGGCAAGACAGT
GTCGCTGTACACCTGCCTGAACCTGCTGAACAAGGCCGGCGTGAACATCGCCACGGCGGAAGATCCTTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAATGTGAACGAAAAGGCCGGCCTCACCTTTGCCACTGCGCTCAAGGCTTTTCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCA
CTTGGTGCTGTCAACGCTGCACACCAATGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCA
ACATCGCATCGAGCGTGATCCTGATCACCGCCCAGCGCCTGGCCAGGCGCCTGTGCCCTCAATGCAAGGAGCCGGCTGAC
ATTCCGCGGGAGGCCCTGATCGATGCAGGCTACGACGATGCGGACATTGACGGCAGCTGGGTCACCTACAGGCCCGTCGG
CTGTCCGGCATGCAACAACGGCTACAAGGGCCGCGTAGGCATCTATCAGGTGATGCCTATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAGGACGGCAGTGCGCTGGAGATTGCCGCACAGGCCAAGCGCGAAGGCGTGCGGTCGCTGCGTGGATCGGGT
TTGCACAAGGCCAGACAGGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.206

97.241

0.547
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

49.736

98.103

0.488
  pilB Vibrio cholerae strain A1552

46.679

96.034

0.448
  pilB Vibrio campbellii strain DS40M4

44.248

97.414

0.431
  pilB Vibrio parahaemolyticus RIMD 2210633

47.036

90.172

0.424
  pilF Thermus thermophilus HB27

38.889

99.31

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

83.793

0.362

Multiple sequence alignment