Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U6115_RS05115 Genome accession   NZ_CP142381
Coordinates   1062689..1064395 (+) Length   568 a.a.
NCBI ID   WP_043578580.1    Uniprot ID   A0A2R2IW97
Organism   Chromobacterium subtsugae strain ATCC 31532     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1057689..1069395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U6115_RS05095 (U6115_05095) ffh 1058239..1059594 (-) 1356 WP_043578569.1 signal recognition particle protein -
  U6115_RS05100 (U6115_05100) - 1059710..1060531 (+) 822 WP_043578572.1 inner membrane protein YpjD -
  U6115_RS05105 (U6115_05105) comE 1060592..1060891 (+) 300 WP_043578575.1 ComEA family DNA-binding protein Machinery gene
  U6115_RS05110 (U6115_05110) - 1061308..1062489 (+) 1182 WP_043578577.1 porin -
  U6115_RS05115 (U6115_05115) pilF 1062689..1064395 (+) 1707 WP_043578580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U6115_RS05120 (U6115_05120) pilC 1064448..1065671 (+) 1224 WP_043578583.1 type II secretion system F family protein Machinery gene
  U6115_RS05125 (U6115_05125) - 1065671..1066591 (+) 921 WP_043578586.1 prepilin peptidase -
  U6115_RS05130 (U6115_05130) coaE 1066576..1067190 (+) 615 WP_043578588.1 dephospho-CoA kinase -
  U6115_RS05135 (U6115_05135) zapD 1067226..1067984 (+) 759 WP_043578820.1 cell division protein ZapD -
  U6115_RS05140 (U6115_05140) yacG 1067990..1068184 (+) 195 WP_043578592.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62605.07 Da        Isoelectric Point: 5.5749

>NTDB_id=925616 U6115_RS05115 WP_043578580.1 1062689..1064395(+) (pilF) [Chromobacterium subtsugae strain ATCC 31532]
MEASAGISGLGRALVQHNMLLQQDADAIMRQAGASHITFVEQLILSKKMSARDIAVFASRVFGYPLLDMSSLDTEQLPKG
LIDDELIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEIVVVEDDKLSKTLGRYQDNSTSALKELENEDFG
DLEFADPDAQVDSGPQVEVDDAPVVKFIHKMLLDAIAGSASDIHFEPYEKYYRIRYRIDGELKEVAQPPLILKEKIASRI
KVISKLDISEKRLPQDGRLKLVISKSRAIDFRVSTLPTLYGEKIVMRILDSSAATLDIEQLGFEPEQKQLLMTTIARPYG
MVLVTGPTGSGKTVSLYTFLNLLNRPDTNISTAEDPVEINLPGINQVNVNERAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKKPVDIPEEALLRAGFRK
EDLDGSWQPYGPVGCEECRNTGYRGRTGIYEVMPITDAMTRLIMKNGTAVDISDLALQEGMVDLRHAGLLKVKRGVTSLA
EIEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=925616 U6115_RS05115 WP_043578580.1 1062689..1064395(+) (pilF) [Chromobacterium subtsugae strain ATCC 31532]
ATGGAAGCTTCGGCAGGGATTTCCGGCCTGGGTAGGGCCCTGGTTCAGCACAATATGCTGCTGCAGCAGGACGCGGACGC
CATCATGCGCCAGGCGGGCGCTTCGCATATCACTTTTGTCGAGCAACTGATACTCAGCAAGAAAATGAGCGCGCGGGACA
TCGCGGTATTCGCGTCGCGGGTTTTCGGCTACCCCTTGCTGGACATGAGCTCTCTGGATACCGAACAGCTGCCCAAGGGG
CTGATCGACGACGAGTTGATCGCCAGCCGGCGCCTGCTGCCGCTGCTCAAGCGCGGCAACAAGCTCTATATCGGCACTTC
CGACCCCACCCAGACTTCCGCTTATCACGCGATCACCTTCAAGACCGGTCTGACGGTGGAAATCGTCGTGGTCGAGGATG
ACAAGCTGTCCAAGACGCTGGGACGCTATCAGGACAATTCCACCTCGGCGCTGAAGGAGCTGGAAAACGAGGATTTCGGC
GACCTGGAGTTCGCCGATCCGGACGCCCAGGTAGACAGCGGTCCCCAGGTGGAGGTGGACGACGCGCCGGTGGTCAAATT
CATCCACAAGATGCTGCTGGACGCCATTGCCGGCAGCGCGTCGGACATCCACTTCGAACCGTATGAAAAGTATTACCGCA
TACGCTACCGGATAGACGGCGAGTTGAAGGAGGTGGCGCAGCCGCCGCTGATCCTGAAGGAAAAGATCGCCTCGCGCATC
AAGGTGATTTCCAAGCTCGACATTTCGGAAAAGCGTCTGCCGCAGGATGGCCGCCTCAAGCTGGTGATCTCCAAGAGCCG
CGCCATCGATTTCCGCGTCAGCACGCTGCCGACGCTGTATGGCGAAAAGATCGTGATGCGGATACTGGATTCGTCGGCGG
CGACGCTGGACATCGAGCAGCTGGGCTTCGAGCCGGAACAAAAACAGCTGCTGATGACGACGATAGCGCGTCCCTACGGC
ATGGTGCTGGTGACCGGGCCGACCGGCAGCGGCAAAACGGTGTCGCTGTATACTTTCCTTAACCTTTTGAACCGGCCGGA
CACCAATATTTCCACCGCGGAGGACCCGGTGGAGATCAACCTGCCCGGCATCAACCAGGTCAACGTCAACGAGCGGGCGG
GCCTGACTTTCGCTTCGGCGCTGAAGGCTTTCCTGCGGCAGGACCCGGATGTGATCATGGTGGGCGAGATCCGCGACTTT
GAAACCGCCGACATCGCGATCAAGGCGGCGCAGACCGGCCATATGGTATTTTCCACCCTGCACACCAATAATGCGCCGGC
GACGCTGACCCGGATGCTGAACATGGGGGTGGCGCCGTTCAATATCGCCAGCTCGGTGCTGCTGATCATGGCGCAGCGGC
TGGCGCGCCGCCTGTGCAGCCATTGCAAGAAGCCGGTCGACATTCCGGAGGAGGCGCTGCTGCGCGCCGGCTTTCGCAAG
GAAGACCTGGACGGCAGCTGGCAGCCCTATGGCCCGGTCGGCTGCGAGGAGTGCCGCAATACCGGCTATCGCGGGCGCAC
CGGCATTTACGAGGTGATGCCGATCACCGACGCGATGACGCGGCTGATCATGAAAAACGGCACCGCGGTCGATATTTCCG
ACCTGGCCCTGCAGGAGGGCATGGTTGATTTGCGCCACGCCGGCTTGCTCAAGGTCAAGCGCGGGGTGACCTCGCTGGCG
GAAATCGAAGCGGTGACCAACGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R2IW97

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.584

98.944

0.56
  pilB Acinetobacter baumannii D1279779

54.007

100

0.546
  pilB Acinetobacter baylyi ADP1

54.707

99.12

0.542
  pilB Legionella pneumophila strain ERS1305867

53.054

100

0.535
  pilB Vibrio cholerae strain A1552

48.932

98.944

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

98.944

0.472
  pilB Vibrio campbellii strain DS40M4

46.181

99.12

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.226

93.662

0.377
  pilF Thermus thermophilus HB27

37.722

98.944

0.373