Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VKP36_RS00675 Genome accession   NZ_CP142357
Coordinates   104755..105189 (+) Length   144 a.a.
NCBI ID   WP_326624919.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain AW-534     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99755..110189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP36_RS00645 (VKP36_00645) - 101424..101789 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP36_RS00650 (VKP36_00650) comYA 101882..102820 (+) 939 WP_129304381.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP36_RS00655 (VKP36_00655) comYB 102756..103790 (+) 1035 WP_227868552.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP36_RS00660 (VKP36_00660) comYC 103792..104118 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP36_RS00665 (VKP36_00665) comGD 104093..104521 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  VKP36_RS00670 (VKP36_00670) comGE 104478..104762 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  VKP36_RS00675 (VKP36_00675) comYF 104755..105189 (+) 435 WP_326624919.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP36_RS00680 (VKP36_00680) comGG 105173..105499 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  VKP36_RS00685 (VKP36_00685) comYH 105597..106550 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  VKP36_RS00690 (VKP36_00690) - 106609..107805 (+) 1197 WP_030126459.1 acetate kinase -
  VKP36_RS00695 (VKP36_00695) - 107991..108299 (+) 309 WP_030126458.1 hypothetical protein -
  VKP36_RS00700 (VKP36_00700) proC 108382..109152 (-) 771 WP_002992745.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16679.28 Da        Isoelectric Point: 10.2526

>NTDB_id=925357 VKP36_RS00675 WP_326624919.1 104755..105189(+) (comYF) [Streptococcus pyogenes strain AW-534]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTFLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=925357 VKP36_RS00675 WP_326624919.1 104755..105189(+) (comYF) [Streptococcus pyogenes strain AW-534]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCTTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae D39

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae R6

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae TIGR4

42.857

92.361

0.396
  comGF/cglF Streptococcus mitis SK321

42.105

92.361

0.389