Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VKP35_RS00800 Genome accession   NZ_CP142349
Coordinates   121786..122220 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 11RS100     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 116786..127220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VKP35_RS00770 (VKP35_00770) - 118455..118820 (+) 366 WP_002986560.1 DUF1033 family protein -
  VKP35_RS00775 (VKP35_00775) comYA 118913..119851 (+) 939 WP_009880361.1 competence type IV pilus ATPase ComGA Machinery gene
  VKP35_RS00780 (VKP35_00780) comYB 119787..120821 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  VKP35_RS00785 (VKP35_00785) comYC 120823..121149 (+) 327 WP_111713112.1 competence type IV pilus major pilin ComGC Machinery gene
  VKP35_RS00790 (VKP35_00790) comGD 121124..121552 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  VKP35_RS00795 (VKP35_00795) comGE 121509..121793 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  VKP35_RS00800 (VKP35_00800) comYF 121786..122220 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  VKP35_RS00805 (VKP35_00805) comGG 122204..122530 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  VKP35_RS00810 (VKP35_00810) comYH 122628..123581 (+) 954 WP_032467307.1 class I SAM-dependent methyltransferase Machinery gene
  VKP35_RS00815 (VKP35_00815) - 123640..124836 (+) 1197 WP_002986533.1 acetate kinase -
  VKP35_RS00820 (VKP35_00820) - 125023..125331 (+) 309 WP_011017251.1 hypothetical protein -
  VKP35_RS00825 (VKP35_00825) proC 125414..126184 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=924920 VKP35_RS00800 WP_002986542.1 121786..122220(+) (comYF) [Streptococcus pyogenes strain 11RS100]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=924920 VKP35_RS00800 WP_002986542.1 121786..122220(+) (comYF) [Streptococcus pyogenes strain 11RS100]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396