Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV49_RS01165 Genome accession   NZ_AP025499
Coordinates   258375..258986 (+) Length   203 a.a.
NCBI ID   WP_192853349.1    Uniprot ID   -
Organism   Vibrio lentus strain LMG 21034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 253375..263986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV49_RS01150 - 254049..256607 (-) 2559 WP_065104131.1 PBP1A family penicillin-binding protein -
  OCV49_RS01155 pilM 256759..257784 (+) 1026 WP_102296354.1 type IV pilus assembly protein PilM Machinery gene
  OCV49_RS01160 pilN 257768..258382 (+) 615 WP_192853348.1 PilN domain-containing protein Machinery gene
  OCV49_RS01165 pilO 258375..258986 (+) 612 WP_192853349.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV49_RS01170 pilP 258973..259491 (+) 519 WP_076668786.1 pilus assembly protein PilP Machinery gene
  OCV49_RS01175 pilQ 259551..261245 (+) 1695 WP_102279228.1 type IV pilus secretin PilQ family protein Machinery gene
  OCV49_RS01180 aroK 261422..261940 (+) 519 WP_004729664.1 shikimate kinase AroK -
  OCV49_RS01185 aroB 261967..263055 (+) 1089 WP_192853350.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23470.94 Da        Isoelectric Point: 4.3571

>NTDB_id=92172 OCV49_RS01165 WP_192853349.1 258375..258986(+) (pilO) [Vibrio lentus strain LMG 21034]
MASFQNMVSLQDLDVDEITEWPLLPQLAVVLLLMILIQGVGVWFYIMPMDDELQQLKQQEQTLKATLRIKANKVAALPKL
QSLLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNALTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGDFSAAIA
KLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVDDETQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=92172 OCV49_RS01165 WP_192853349.1 258375..258986(+) (pilO) [Vibrio lentus strain LMG 21034]
ATGGCTAGTTTTCAAAATATGGTCAGTTTGCAAGACCTTGATGTCGATGAGATCACTGAATGGCCGCTACTGCCTCAACT
GGCGGTGGTTCTATTGCTGATGATCTTGATTCAGGGCGTTGGCGTTTGGTTCTACATCATGCCAATGGATGATGAACTGC
AACAATTGAAGCAGCAAGAGCAGACCTTGAAAGCCACTTTAAGGATTAAAGCCAATAAGGTCGCAGCTCTACCGAAGCTT
CAGAGCCTGTTGGATGAGCTGACTAGCCGTTACGATTATCTGTTAGAGCAATTACCCGTGCAAAAAGAGCTCGCCAGTAT
GTTGGCTTCCGTCAATGAACTTGGCTTGGATAATGCGCTGACCTTTACTCGAATTGATTGGGGACAAAAGCAGAACAAAG
AGTTTCTTTATCGCTTACCGCTCAATATCGAGCTGACGGGTGACTACCATGAAATTGGTGACTTCTCTGCGGCCATCGCC
AAGCTGCCGCGCATTATTAGCTTCGATGATGTGAATTGGCAGCGAGTCAGTCAAGAAAGCAGCACGTTGCATTTCCGAGT
TCGCGCTTATACCTACCAGTTTAAGTCGGAGGTAGACGATGAAACTCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

77.949

96.059

0.749
  pilO Vibrio campbellii strain DS40M4

72.487

93.103

0.675

Multiple sequence alignment