Detailed information NTG0921625

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: VQ575_RS15680
• Genome accession: NZ_CP142104
• Coordinates: 3557718..3558833 (-)
• Length: 371 a.a.
• NCBI ID: WP_039590036.1
• Uniprot ID: A0A0B1Z2U8
• Organism: Pseudomonas frederiksbergensis strain YE17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3552718..3563833

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VQ575_RS15665 (VQ575_15665)	-	3553448..3554839 (+)	1392	WP_039589505.1	GntP family permease	-
VQ575_RS15670 (VQ575_15670)	hbdH	3554847..3555623 (+)	777	WP_039589503.1	3-hydroxybutyrate dehydrogenase	-
VQ575_RS15675 (VQ575_15675)	-	3555724..3557679 (+)	1956	WP_039589502.1	acetoacetate--CoA ligase	-
VQ575_RS15680 (VQ575_15680)	pilU	3557718..3558833 (-)	1116	WP_039590036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VQ575_RS15685 (VQ575_15685)	-	3558966..3561503 (+)	2538	WP_325917900.1	PAS domain-containing protein	-
VQ575_RS15690 (VQ575_15690)	-	3561552..3561827 (+)	276	WP_019690952.1	peptidylprolyl isomerase	-
VQ575_RS15695 (VQ575_15695)	-	3561883..3562797 (-)	915	WP_325917902.1	sugar kinase	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41183.17 Da
• Isoelectric Point: 6.1928

>NTDB_id=921625 VQ575_RS15680 WP_039590036.1 3557718..3558833(-) (pilU) [Pseudomonas frederiksbergensis strain YE17]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVLKPLSNQAFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPKVLLETIMHKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLQDLGNNLKAFVSQRLVRTRAGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAAPPAEPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=921625 VQ575_RS15680 WP_039590036.1 3557718..3558833(-) (pilU) [Pseudomonas frederiksbergensis strain YE17]
ATGGAAATTGATCCTTTATTACGAATCCTGGCAAGCCAGGACGGCTCCGACCTCTACATGTCCACCGGCGCACCGCCCTG
CGCGCGTTTCGAAGGTGTGCTCAAGCCGCTGAGCAACCAGGCGTTCAAGGTCGGCGATATCGCCGGGCTTGCCGAGTCAT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATTTCCTTGGCGGGGGTCGGACGG
TTCCGCGTCAACATCTTCAAGCAGCGCAATGACGTGTCGATGGTCATACGCAACGTCAAGCTGGATATTCCACGCTTCGA
AGACCTGAAGTTACCGAAGGTGCTGCTGGAAACCATCATGCACAAGCAAGGGCTGATGCTGTTCGTCGGCGCCACGGGCT
CGGGCAAATCGACGTCCCTGGCGGCGCTGATCGATTACCGAAACCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAATATATTCACCGGCACAAGAAATCCATCGTCAACCAGCGAGAGGTCGGCGTGGATACCCGCAGTTTCCATGC
TGCCCTCAAGAACACTTTGCGCCAGGCGCCTGACGTGGTGCTGATCGGTGAAATCCGCGACCGCGAGACCATGGAACACG
CGTTGGCCTTCGCTGACACCGGCCACTTGGTGATTTCCACCTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTTTTCCCGGAAGAACGCCGGCCGCAACTGCTGCAGGATCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTGCGCACCCGCGCCGGCCAGCGGCGGGCGGCGGTGGAAGTGATGCTTGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAGCTGGGCGAGCTCAAGGGCATTATGGAAAAGTCCGAAGAACTGGGGATGCAGACGTTCGACCAGGCATTG
TTCAATCTGGTGGTGGAAGGTGCGATCGACGAAGAAGAAGCGCTGAAAAACGCGGACTCGGCGAACAATTTACGCTTGCG
GCTGAAGTTGCATGCGGAGTCGGGCGCGGCGCCACCGGCTGAGCCGGCGGCGGGGGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

3D structure

• AlphaFold DB: A0A0B1Z2U8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.901	95.687	0.544
pilU	Acinetobacter baylyi ADP1	52.101	96.226	0.501
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.108	99.73	0.38
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364