Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV56_RS14735 Genome accession   NZ_AP025492
Coordinates   3273527..3274138 (-) Length   203 a.a.
NCBI ID   WP_086715787.1    Uniprot ID   A0A5M9NBH1
Organism   Vibrio gigantis strain LMG 22741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3268527..3279138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS14715 aroB 3269459..3270547 (-) 1089 WP_086715777.1 3-dehydroquinate synthase -
  OCV56_RS14720 aroK 3270573..3271091 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OCV56_RS14725 pilQ 3271268..3272962 (-) 1695 WP_190960479.1 type IV pilus secretin PilQ Machinery gene
  OCV56_RS14730 pilP 3273022..3273540 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  OCV56_RS14735 pilO 3273527..3274138 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV56_RS14740 pilN 3274131..3274730 (-) 600 WP_086715791.1 PilN domain-containing protein Machinery gene
  OCV56_RS14745 pilM 3274714..3275739 (-) 1026 WP_086715793.1 type IV pilus assembly protein PilM Machinery gene
  OCV56_RS14750 - 3275891..3278449 (+) 2559 WP_167373191.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23573.00 Da        Isoelectric Point: 4.4907

>NTDB_id=92111 OCV56_RS14735 WP_086715787.1 3273527..3274138(-) (pilO) [Vibrio gigantis strain LMG 22741]
MANLQNRMSFQDLDVDEITEWPLLPQLLVILVLVVLIQGIGTWLYVLPLDDELQQMKQQEQTLKTTLRIKANKVAALPKL
QSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNSLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGDFSAAIA
KLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDEIQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=92111 OCV56_RS14735 WP_086715787.1 3273527..3274138(-) (pilO) [Vibrio gigantis strain LMG 22741]
ATGGCTAACCTTCAAAACAGGATGAGTTTTCAGGATCTTGATGTTGATGAGATCACCGAGTGGCCGCTGTTGCCTCAACT
CCTAGTCATTCTGGTGTTGGTAGTATTAATCCAAGGTATCGGAACCTGGCTTTATGTGCTGCCGCTCGACGATGAACTGC
AACAGATGAAGCAGCAAGAGCAGACCTTAAAAACGACCTTGCGAATAAAGGCCAACAAGGTTGCGGCTCTGCCTAAGTTA
CAGAGCCAGCTGGATGAGCTAACCAGTCGCTACGATTACCTGTTAGAGCAGCTCCCTGTTCAAAAAGAGCTAGCCAGTAT
GTTGGCGTCAGTGAATGAACTCGGCTTGGATAATTCGCTGACTTTCACTCGAATCGATTGGGGACAAAAGCAGAACAAGG
AGTTTCTCTATCGTTTACCGCTCAATATCGAGTTGACAGGTGACTACCACGAGATAGGCGACTTCTCTGCGGCTATCGCC
AAGCTGCCGCGTATCATCAGCTTTGATGATGTGAATTGGCAACGAGTCAGCCAAGAAAGCAGCACGCTGCACTTTAGGGT
TCGAGCTTATACCTACCAGTTTAAGTCGGAGGTTAATGATGAAATCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9NBH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.648

95.074

0.719
  pilO Vibrio campbellii strain DS40M4

72.34

92.611

0.67

Multiple sequence alignment