Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OCV56_RS14740 Genome accession   NZ_AP025492
Coordinates   3274131..3274730 (-) Length   199 a.a.
NCBI ID   WP_086715791.1    Uniprot ID   A0A5M9NE71
Organism   Vibrio gigantis strain LMG 22741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3269131..3279730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS14715 aroB 3269459..3270547 (-) 1089 WP_086715777.1 3-dehydroquinate synthase -
  OCV56_RS14720 aroK 3270573..3271091 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OCV56_RS14725 pilQ 3271268..3272962 (-) 1695 WP_190960479.1 type IV pilus secretin PilQ Machinery gene
  OCV56_RS14730 pilP 3273022..3273540 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  OCV56_RS14735 pilO 3273527..3274138 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV56_RS14740 pilN 3274131..3274730 (-) 600 WP_086715791.1 PilN domain-containing protein Machinery gene
  OCV56_RS14745 pilM 3274714..3275739 (-) 1026 WP_086715793.1 type IV pilus assembly protein PilM Machinery gene
  OCV56_RS14750 - 3275891..3278449 (+) 2559 WP_167373191.1 penicillin-binding protein 1A -
  OCV56_RS14755 oxyR 3278543..3279436 (-) 894 WP_086715797.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22916.30 Da        Isoelectric Point: 9.6558

>NTDB_id=92112 OCV56_RS14740 WP_086715791.1 3274131..3274730(-) (pilN) [Vibrio gigantis strain LMG 22741]
MLHQINLLPWRDEIRTQHKKRFIHLVILGVIIALGGQWAVGNYFHDQQTKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSESLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLESTSASRKGKEANHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=92112 OCV56_RS14740 WP_086715791.1 3274131..3274730(-) (pilN) [Vibrio gigantis strain LMG 22741]
ATGTTGCATCAAATTAACTTGCTTCCTTGGCGTGATGAGATTCGCACTCAACACAAGAAGCGTTTTATCCACTTGGTCAT
TCTTGGTGTCATTATTGCGCTTGGTGGGCAATGGGCGGTTGGTAACTACTTTCACGACCAACAAACCAAGCAGCAAGCGC
GCCTCAACTATCTCAATCAGTATATCGCTGAGCTCGACCGACAAATTCAGTCATTAAAAGTCGCGGAACAAGAGCACAAA
GCCATTCTGACTCGACTTGATGTGGTTGAGTCTTTACAGCTTGGCCGCAATAAGACCACCGACTTTATGAACTTGATGCC
GGAGCTAATTCCTGAGGGCGTGTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGATA
GTACCGCTCGTCTCGCCACCATGTTGGATAACCTAGAGCGTTCTGAATCACTGAAAGGGGTTGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTTAATAAAGAGTTCCAGACCTTCAAGGTCTCTTTTGTGTTCAGTCCAGTTTCTTTAGAAAGTAC
TAGTGCGAGCCGTAAAGGGAAGGAGGCGAATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9NE71

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

61.856

97.487

0.603
  pilN Vibrio campbellii strain DS40M4

63.978

93.467

0.598

Multiple sequence alignment