Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OCV56_RS14745 Genome accession   NZ_AP025492
Coordinates   3274714..3275739 (-) Length   341 a.a.
NCBI ID   WP_086715793.1    Uniprot ID   A0A5M9NEZ5
Organism   Vibrio gigantis strain LMG 22741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3269714..3280739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS14720 aroK 3270573..3271091 (-) 519 WP_009848907.1 shikimate kinase AroK -
  OCV56_RS14725 pilQ 3271268..3272962 (-) 1695 WP_190960479.1 type IV pilus secretin PilQ Machinery gene
  OCV56_RS14730 pilP 3273022..3273540 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  OCV56_RS14735 pilO 3273527..3274138 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV56_RS14740 pilN 3274131..3274730 (-) 600 WP_086715791.1 PilN domain-containing protein Machinery gene
  OCV56_RS14745 pilM 3274714..3275739 (-) 1026 WP_086715793.1 type IV pilus assembly protein PilM Machinery gene
  OCV56_RS14750 - 3275891..3278449 (+) 2559 WP_167373191.1 penicillin-binding protein 1A -
  OCV56_RS14755 oxyR 3278543..3279436 (-) 894 WP_086715797.1 DNA-binding transcriptional regulator OxyR -
  OCV56_RS14760 - 3279657..3280385 (+) 729 WP_048659171.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38374.87 Da        Isoelectric Point: 6.7239

>NTDB_id=92113 OCV56_RS14745 WP_086715793.1 3274714..3275739(-) (pilM) [Vibrio gigantis strain LMG 22741]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKELPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQIYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPEKKNWLLVDVGIDQTSLGIIPQGSVPFYKDIGFGTKDLRSHDDPSDMECAFGTTEDTH
QFIVNLIQKLKRQLQLYSSGHAQQSISGIWLMGEGASIPMVTEELERHFQMSCESLNPLSLFENKVAKRRRLSMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=92113 OCV56_RS14745 WP_086715793.1 3274714..3275739(-) (pilM) [Vibrio gigantis strain LMG 22741]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGAACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATTGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGCAAAGGTTCGACAA
GCAGTTATCAAATATACGCAACTCGTAAGGAAGTGGTGGATAGCCGAGCGGATGCATTAACTAAGGCTGGCTTTAAGCCT
GTTGTGGTGGATACGCAAGCGCATGGGTTGCTTAATATCTGGCAATTGGCCTCGCGCCTGTATCCTGAAAAGAAAAACTG
GCTGCTGGTGGATGTTGGAATCGATCAAACGTCGCTAGGTATTATTCCACAAGGTTCGGTACCGTTTTACAAAGACATCG
GTTTTGGTACCAAAGATCTTCGTAGTCATGACGACCCTAGTGATATGGAGTGTGCTTTCGGCACCACGGAAGACACACAT
CAATTCATTGTTAACCTGATTCAGAAGCTCAAACGTCAATTGCAGCTCTATTCTTCGGGACATGCGCAGCAATCTATCTC
TGGGATCTGGCTGATGGGTGAAGGCGCCAGCATCCCTATGGTAACCGAAGAGCTAGAACGCCATTTTCAGATGAGCTGCG
AGTCATTGAATCCTTTGTCGTTGTTTGAGAATAAGGTTGCCAAGAGACGCCGACTGTCGATGGACTGGCAACACTTTGGT
ATTGCGGCAGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9NEZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

53.982

99.413

0.537
  pilM Vibrio campbellii strain DS40M4

48.116

100

0.487

Multiple sequence alignment