Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VN992_RS04680 Genome accession   NZ_CP141949
Coordinates   1027693..1029087 (-) Length   464 a.a.
NCBI ID   WP_370427297.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain AH2022     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022693..1034087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN992_RS04650 (VN992_04645) lptE 1023305..1023799 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  VN992_RS04655 (VN992_04650) holA 1023908..1024933 (+) 1026 WP_012263005.1 DNA polymerase III subunit delta -
  VN992_RS04660 (VN992_04655) yacG 1025010..1025198 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  VN992_RS04665 (VN992_04660) coaE 1025180..1025818 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  VN992_RS04670 (VN992_04665) - 1025840..1026478 (-) 639 WP_370427325.1 prepilin peptidase -
  VN992_RS04675 (VN992_04670) - 1026505..1027709 (-) 1205 Protein_912 type II secretion system F family protein -
  VN992_RS04680 (VN992_04675) pilB 1027693..1029087 (-) 1395 WP_370427297.1 GspE/PulE family protein Machinery gene
  VN992_RS04685 (VN992_04680) pilA 1029105..1029551 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN992_RS04690 (VN992_04685) radA 1029733..1031112 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  VN992_RS04695 (VN992_04690) - 1031156..1031389 (-) 234 WP_005597399.1 YgjV family protein -
  VN992_RS04700 (VN992_04695) - 1031516..1032004 (-) 489 WP_370427298.1 dihydrofolate reductase -
  VN992_RS04705 (VN992_04700) proB 1032091..1033182 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  VN992_RS04710 (VN992_04705) - 1033195..1033971 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52992.87 Da        Isoelectric Point: 6.5042

>NTDB_id=920569 VN992_RS04680 WP_370427297.1 1027693..1029087(-) (pilB) [Actinobacillus pleuropneumoniae strain AH2022]
MQYSVCDVNNRQVFRISEEQWQKNYDERHILLRYLTVPLREDQQTLWLAIDDMNNLSACEIFAFLTHKRIEPVLVSSEEL
KYLLGQLMPEQQSVYDETELPYQPLAQAQHSDVNDPIIQVLDGLFKYGLKHNASDIHLEPQKDKMLIRLRIDGVLHPYKS
FSLQLANRLISRIKLLAKLDISESRLPQDGQFGFKTILSETLDFRVSSLPTYWGEKVVLRLQKNKPTHFDFTTLGFLAEQ
KEKLLRALRQPQGLILVTGPTGSGKSITLYSALDYLNDSKRHILTAEDPIEIELEGIIQTQINPAIDLGFSRLLRTFLRQ
DPDVIMLGEIRDQESAEIALSAAQTGHLVLSTLHTNDAPSAIERLLQLGIKEYEINNSLLLVVAQRLVRKLCTECGGKGC
KYCYHGYRGRIGVYQLFSKNGKIFEKSTACLDFENLYQSAQQKFKAGITSLEEIERVLGHVESV

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=920569 VN992_RS04680 WP_370427297.1 1027693..1029087(-) (pilB) [Actinobacillus pleuropneumoniae strain AH2022]
ATGCAGTATTCCGTATGTGATGTAAACAATCGGCAAGTATTCCGGATTTCCGAGGAACAGTGGCAAAAAAATTATGATGA
ACGTCATATCTTATTACGTTATCTCACCGTACCGCTTCGGGAAGACCAACAAACATTATGGTTGGCAATCGATGATATGA
ATAATCTCAGTGCTTGCGAGATTTTTGCTTTTCTTACGCATAAGCGAATTGAGCCGGTATTAGTCTCTTCCGAAGAATTG
AAATACCTGCTCGGGCAATTAATGCCGGAACAACAATCGGTATATGACGAAACGGAATTACCTTATCAACCTTTAGCACA
AGCGCAGCATTCGGATGTTAATGATCCGATTATTCAAGTGTTGGACGGCCTGTTTAAATACGGTTTAAAACATAATGCTT
CGGATATTCATTTGGAACCGCAAAAAGACAAAATGTTGATTCGATTACGTATCGACGGCGTGTTACATCCGTATAAATCA
TTTTCTCTCCAACTTGCCAATCGACTTATTTCTCGCATTAAATTATTAGCTAAACTGGATATTAGTGAATCCCGTCTTCC
GCAAGACGGGCAATTCGGTTTTAAGACAATCTTATCCGAAACATTGGATTTCCGTGTTTCGAGTTTACCGACTTATTGGG
GCGAAAAAGTCGTTTTACGGCTACAAAAAAATAAACCGACGCATTTTGATTTTACTACGTTGGGCTTCTTGGCGGAGCAG
AAGGAAAAGTTATTGCGGGCATTGCGGCAGCCTCAAGGATTAATATTAGTTACGGGACCGACCGGCAGTGGTAAAAGTAT
TACCTTATATAGTGCGTTGGATTATTTAAATGACAGTAAAAGGCATATTTTGACCGCCGAAGATCCGATAGAAATCGAAC
TTGAAGGCATAATCCAAACGCAAATAAACCCAGCGATAGATTTAGGGTTCAGCCGGCTATTAAGAACGTTTTTACGACAA
GATCCGGATGTGATTATGTTAGGTGAAATTCGTGATCAAGAAAGTGCCGAAATTGCATTAAGTGCGGCACAAACCGGGCA
TTTAGTGCTTTCGACCTTACATACCAATGATGCGCCTTCGGCAATCGAACGCTTATTGCAGCTCGGTATCAAAGAATATG
AAATCAATAATTCGTTATTACTTGTCGTGGCGCAACGTTTAGTTCGTAAATTATGTACCGAATGCGGCGGAAAAGGCTGT
AAATATTGTTATCATGGCTACAGAGGCAGAATCGGCGTTTATCAACTGTTTTCAAAAAATGGAAAAATTTTTGAGAAATC
GACCGCTTGTTTGGATTTTGAAAACTTATATCAAAGTGCGCAACAGAAATTCAAGGCGGGAATCACTTCATTAGAAGAAA
TCGAGAGAGTGTTAGGTCATGTTGAAAGTGTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(28-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

68.192

98.922

0.675
  pilB Haemophilus influenzae Rd KW20

58.72

97.629

0.573
  pilB Haemophilus influenzae 86-028NP

58.278

97.629

0.569
  pilB Vibrio campbellii strain DS40M4

40.125

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.264

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.412
  pilB Acinetobacter baumannii D1279779

38.855

100

0.409
  pilB Vibrio cholerae strain A1552

39.004

100

0.405
  pilB Acinetobacter baylyi ADP1

38.32

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.538

100

0.369