Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VN992_RS04685 Genome accession   NZ_CP141949
Coordinates   1029105..1029551 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain AH2022     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024105..1034551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VN992_RS04660 (VN992_04655) yacG 1025010..1025198 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  VN992_RS04665 (VN992_04660) coaE 1025180..1025818 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  VN992_RS04670 (VN992_04665) - 1025840..1026478 (-) 639 WP_370427325.1 prepilin peptidase -
  VN992_RS04675 (VN992_04670) - 1026505..1027709 (-) 1205 Protein_912 type II secretion system F family protein -
  VN992_RS04680 (VN992_04675) pilB 1027693..1029087 (-) 1395 WP_370427297.1 GspE/PulE family protein Machinery gene
  VN992_RS04685 (VN992_04680) pilA 1029105..1029551 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VN992_RS04690 (VN992_04685) radA 1029733..1031112 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  VN992_RS04695 (VN992_04690) - 1031156..1031389 (-) 234 WP_005597399.1 YgjV family protein -
  VN992_RS04700 (VN992_04695) - 1031516..1032004 (-) 489 WP_370427298.1 dihydrofolate reductase -
  VN992_RS04705 (VN992_04700) proB 1032091..1033182 (+) 1092 WP_005597401.1 glutamate 5-kinase -
  VN992_RS04710 (VN992_04705) - 1033195..1033971 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=920570 VN992_RS04685 WP_005597397.1 1029105..1029551(-) (pilA) [Actinobacillus pleuropneumoniae strain AH2022]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=920570 VN992_RS04685 WP_005597397.1 1029105..1029551(-) (pilA) [Actinobacillus pleuropneumoniae strain AH2022]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527