Detailed information NTG0919808

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: VO180_RS07400
• Genome accession: NZ_CP141826
• Coordinates: 1463905..1464519 (+)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain XHA16
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1458905..1469519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VO180_RS07365 (VO180_07365)	aroE	1459592..1460434 (+)	843	WP_324636675.1	shikimate dehydrogenase	-
VO180_RS07370 (VO180_07370)	yhbY	1460428..1460718 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
VO180_RS07375 (VO180_07375)	-	1460729..1461298 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
VO180_RS07380 (VO180_07380)	yqeK	1461288..1461848 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
VO180_RS07385 (VO180_07385)	rsfS	1461866..1462222 (+)	357	WP_007408260.1	ribosome silencing factor	-
VO180_RS07390 (VO180_07390)	-	1462219..1462956 (+)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
VO180_RS07395 (VO180_07395)	comER	1463025..1463846 (-)	822	WP_015417855.1	late competence protein ComER	-
VO180_RS07400 (VO180_07400)	comEA	1463905..1464519 (+)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
VO180_RS07405 (VO180_07405)	-	1464586..1465155 (+)	570	WP_003152868.1	ComE operon protein 2	-
VO180_RS07410 (VO180_07410)	comEC	1465156..1467507 (+)	2352	WP_324636680.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
VO180_RS07415 (VO180_07415)	-	1467526..1467660 (-)	135	WP_003152870.1	YqzM family protein	-
VO180_RS07420 (VO180_07420)	-	1467701..1467852 (+)	152	Protein_1448	hypothetical protein	-
VO180_RS07425 (VO180_07425)	holA	1467892..1468933 (+)	1042	Protein_1449	DNA polymerase III subunit delta	-
VO180_RS07430 (VO180_07430)	rpsT	1468950..1469216 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=919808 VO180_RS07400 WP_053573180.1 1463905..1464519(+) (comEA) [Bacillus velezensis strain XHA16]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=919808 VO180_RS07400 WP_053573180.1 1463905..1464519(+) (comEA) [Bacillus velezensis strain XHA16]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363