Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV52_RS12975 Genome accession   NZ_AP025465
Coordinates   2854961..2856646 (+) Length   561 a.a.
NCBI ID   WP_137409078.1    Uniprot ID   A0A7V7NPI8
Organism   Vibrio chagasii strain LMG 21353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2849961..2861646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV52_RS12955 pdhR 2851424..2852185 (-) 762 WP_004738167.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCV52_RS12960 ampD 2852669..2853268 (-) 600 WP_137409076.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV52_RS12965 nadC 2853352..2854242 (+) 891 WP_137409077.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV52_RS12970 - 2854497..2854961 (+) 465 WP_137409082.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OCV52_RS12975 pilB 2854961..2856646 (+) 1686 WP_137409078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV52_RS12980 pilC 2856662..2857891 (+) 1230 WP_137409079.1 type II secretion system F family protein Machinery gene
  OCV52_RS12985 pilD 2857958..2858827 (+) 870 WP_137409080.1 A24 family peptidase Machinery gene
  OCV52_RS12990 coaE 2858830..2859444 (+) 615 WP_061032470.1 dephospho-CoA kinase -
  OCV52_RS12995 zapD 2859471..2860211 (+) 741 WP_061032469.1 cell division protein ZapD -
  OCV52_RS13000 yacG 2860292..2860489 (+) 198 WP_008222354.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62028.79 Da        Isoelectric Point: 4.9794

>NTDB_id=91740 OCV52_RS12975 WP_137409078.1 2854961..2856646(+) (pilB) [Vibrio chagasii strain LMG 21353]
MLTNLPTILRQADLLSLTQEQAVAEHVQASGISTPEALLVLDLFTGDSLANNIQAIFGLPLVQLSNTDYEALCDQLGLRE
LITKYRALPISVSNSTLTVASADPTDLQAEDDFRFATGLQIELAVANYSELEGAIRKLYGRSISGQESKRKEITQDELAN
LVEVSDDELASIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
IAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSGDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVMPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIAPFNLASSLSLIIAQRLARRLCNQCKTVDDSPDIFLRHSIPNSKT
IYKANPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLISKPNALEIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=91740 OCV52_RS12975 WP_137409078.1 2854961..2856646(+) (pilB) [Vibrio chagasii strain LMG 21353]
ATGCTAACCAACCTCCCTACAATTCTCCGTCAGGCTGATTTACTTAGCCTGACTCAAGAACAAGCCGTGGCGGAGCACGT
GCAAGCTTCAGGTATTTCGACTCCTGAAGCTTTGCTTGTTCTTGATCTCTTCACAGGCGACTCCCTAGCAAACAATATAC
AAGCCATCTTTGGCTTGCCGTTAGTTCAATTGAGCAACACAGATTATGAAGCTTTGTGTGACCAATTAGGGCTTCGCGAG
CTGATAACCAAATACCGTGCACTTCCGATCTCAGTCTCTAATTCGACGCTCACAGTGGCTTCAGCCGACCCTACCGACTT
ACAGGCTGAAGATGATTTTCGTTTTGCTACCGGCCTGCAGATAGAACTGGCAGTCGCCAATTACTCAGAACTGGAAGGCG
CGATACGCAAGTTATACGGCCGTTCTATTTCAGGGCAAGAGTCCAAACGCAAAGAGATCACCCAAGATGAACTGGCTAAC
CTAGTTGAAGTGTCTGACGACGAGCTCGCGTCGATTGAGGACCTTAGCCAAGACGACTCTCCAGTTAGCCGATTTATCAA
TCAGATACTGGTCGATGCGGTACGTAAAGGCGCATCAGATATTCATTTTGAACCTTACGAAGAGCACTACCGTGTGCGCC
TGCGTTGTGACGGTATATTGGTTGAAATCCAGCAACCCGCTTCTCACCTAAGCCGACGTTTATCCGCCCGTTTAAAAATT
ATCGCTAAATTAGATATTGCCGAGCGTCGCTTACCTCAAGACGGACGAATAAAACTTCGCCTGAATGATGAGTTAGCGAT
AGACATGCGCGTCTCCACATTGCCAACCTTGTGGGGCGAGAAGATCGTACTGCGGCTGCTTGATAGCAGCGCGGCGAATC
TCGATATCGACAAGCTCGGGTACAGCGGCGATCAAAAAGCCCTCTACCTGAATGCACTCAAACGCCCGCAAGGAATGATC
TTAATGACAGGCCCCACTGGCAGCGGAAAAACCGTCTCGCTTTATACCGGCCTTAGAGTATTGAACACCACAGAACGTAA
TATCTCCACCGCAGAGGATCCAGTAGAGATTAACCTGTGTGGCATCAATCAAGTGCAAGTGATGCCAAAGATTGGCTTCG
GATTCGCTGAAGCCCTGCGCTCGTTTCTACGACAAGATCCAGATGTGGTGATGGTTGGTGAGATCCGTGATTTAGAAACC
GCAGAAATAGCCATAAAAGCATCGCAAACCGGTCACTTGGTACTTTCAACTCTCCATACCAACTCTGCGGCAGAAACGGT
GACCCGACTCGCTCATATGGGGATTGCGCCCTTTAATCTAGCCTCATCATTGAGCTTAATCATTGCCCAACGACTGGCAA
GACGCTTGTGCAACCAATGCAAAACCGTTGACGACTCTCCAGACATCTTTCTGCGTCACTCAATCCCTAATAGCAAAACC
ATTTATAAGGCCAACCCACAAGGGTGTAACGAGTGCAATCAAGGCTATTCAGGGCGTGTCGGTATTTACGAAGTGATGCC
ATTCACCGACAAACTCAAAAATAGCCTGATCAGCAAACCGAATGCGCTGGAGATTGAAGACCTTGCTCGCCGAGAAGGAA
TGCGCACACTGCAAGAGTCAGGGTTAGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAACTGCAACGTGTTCTGTAC
CTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7V7NPI8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.759

100

0.742
  pilB Vibrio parahaemolyticus RIMD 2210633

70.285

100

0.704
  pilB Vibrio campbellii strain DS40M4

69.751

100

0.699
  pilB Acinetobacter baumannii D1279779

51.341

93.048

0.478
  pilB Legionella pneumophila strain ERS1305867

50.973

91.622

0.467
  pilB Acinetobacter baylyi ADP1

50.386

92.335

0.465
  pilF Neisseria gonorrhoeae MS11

43.034

100

0.435

Multiple sequence alignment