Detailed information NTG0091742

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCV52_RS12985
• Genome accession: NZ_AP025465
• Coordinates: 2857958..2858827 (+)
• Length: 289 a.a.
• NCBI ID: WP_137409080.1
• Uniprot ID: A0A7V7NPR6
• Organism: Vibrio chagasii strain LMG 21353
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2852958..2863827

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCV52_RS12965	nadC	2853352..2854242 (+)	891	WP_137409077.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCV52_RS12970	-	2854497..2854961 (+)	465	WP_137409082.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
OCV52_RS12975	pilB	2854961..2856646 (+)	1686	WP_137409078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OCV52_RS12980	pilC	2856662..2857891 (+)	1230	WP_137409079.1	type II secretion system F family protein	Machinery gene
OCV52_RS12985	pilD	2857958..2858827 (+)	870	WP_137409080.1	A24 family peptidase	Machinery gene
OCV52_RS12990	coaE	2858830..2859444 (+)	615	WP_061032470.1	dephospho-CoA kinase	-
OCV52_RS12995	zapD	2859471..2860211 (+)	741	WP_061032469.1	cell division protein ZapD	-
OCV52_RS13000	yacG	2860292..2860489 (+)	198	WP_008222354.1	DNA gyrase inhibitor YacG	-
OCV52_RS13005	-	2861160..2861966 (-)	807	WP_261900850.1	IS110 family transposase	-
OCV52_RS13010	-	2861988..2862185 (-)	198	WP_261900851.1	hypothetical protein	-
OCV52_RS13015	rplS	2862361..2862714 (-)	354	WP_004738151.1	50S ribosomal protein L19	-
OCV52_RS13020	trmD	2862774..2863514 (-)	741	WP_137408067.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32489.67 Da
• Isoelectric Point: 7.6359

>NTDB_id=91742 OCV52_RS12985 WP_137409080.1 2857958..2858827(+) (pilD) [Vibrio chagasii strain LMG 21353]
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSVPRSTCPKCKTQLRIV
DNIPVLSWLFLKGKCHSCANPISARYPLVELLTATLCTVVASHFGFSFYAIALVFFTFALITATFIDLDTMLLPDQITLP
LVWSGIALALFNIGPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGM

Nucleotide

• Length: 870 bp

>NTDB_id=91742 OCV52_RS12985 WP_137409080.1 2857958..2858827(+) (pilD) [Vibrio chagasii strain LMG 21353]
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCGGTATTAGCATTCATTTTTAGTCTTCTGATTGGCAGCTTCCTTAA
CGTCGTCATACATCGTTTACCAATTATGATGGAACGCGAATGGCAACAAGAGTGCTCGGAGTATTTCTCCCAATATAAAA
TTCCGGCGCCAGAAGGTAAGTTCAACCTCAGTGTTCCTCGCTCTACTTGCCCTAAATGTAAAACTCAATTAAGAATCGTC
GACAATATTCCAGTGTTAAGCTGGTTGTTCTTGAAGGGTAAGTGTCACAGCTGTGCTAATCCAATCAGCGCTCGCTACCC
TTTGGTTGAACTGCTGACTGCGACACTTTGCACAGTTGTTGCCAGTCACTTTGGTTTCAGTTTTTACGCTATTGCACTGG
TATTTTTCACCTTTGCGTTAATTACCGCGACTTTTATCGACCTCGACACCATGCTATTGCCTGATCAAATTACCTTGCCG
TTAGTTTGGTCTGGTATCGCTTTAGCCCTATTTAATATCGGCCCAGTATCGCTTCAAGACTCAGTAGTCGGCGCTATGGC
TGGCTACCTCGCGTTATGGTCCGTCTACTGGCTGTTCAAACTGCTGACAGGTAAAGAAGGCATGGGTTACGGTGACTTCA
AGTTACTGGCTGCACTTGGCGCATGGCTGGGTTGGCAGCATCTACCGATGATCATCCTGCTGTCTTCGCTTGTTGGCCTG
GTGTTTGGTTTGATTCAGCTGCGCTTGAAACAGCAAGGCATTGATAAAGCCTTCCCATTCGGCCCTTACCTTGCGATTGC
TGGTTGGGTAAGCTTGATGTGGGGTAACGACATCATGGGTTGGTATTTCACTTCAGTATTAGGAATGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (136-244)

3D structure

• AlphaFold DB: A0A7V7NPR6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	74.394	100	0.744
pilD	Vibrio cholerae strain A1552	68.403	99.654	0.682
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Acinetobacter baumannii D1279779	50.936	92.388	0.471
pilD	Neisseria gonorrhoeae MS11	46.739	95.502	0.446