Detailed information NTG0916900

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: U4959_RS14845
• Genome accession: NZ_CP141284
• Coordinates: 3075145..3076203 (-)
• Length: 352 a.a.
• NCBI ID: WP_004909635.1
• Uniprot ID: S7Y3V7
• Organism: Acinetobacter junii strain H1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3070145..3081203

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U4959_RS14820 (U4959_14820)	aroK	3070460..3071020 (-)	561	WP_324272892.1	shikimate kinase AroK	-
U4959_RS14825 (U4959_14825)	pilQ	3071040..3073175 (-)	2136	WP_324273067.1	type IV pilus secretin PilQ family protein	Machinery gene
U4959_RS14830 (U4959_14830)	pilP	3073237..3073764 (-)	528	WP_324272893.1	pilus assembly protein PilP	Machinery gene
U4959_RS14835 (U4959_14835)	pilO	3073764..3074504 (-)	741	WP_324272894.1	type 4a pilus biogenesis protein PilO	Machinery gene
U4959_RS14840 (U4959_14840)	pilN	3074501..3075145 (-)	645	WP_324272895.1	PilN domain-containing protein	Machinery gene
U4959_RS14845 (U4959_14845)	pilM	3075145..3076203 (-)	1059	WP_004909635.1	pilus assembly protein PilM	Machinery gene
U4959_RS14850 (U4959_14850)	ponA	3076366..3078945 (+)	2580	WP_004964607.1	penicillin-binding protein PBP1a	-
U4959_RS14855 (U4959_14855)	-	3078960..3079778 (-)	819	WP_004964610.1	putative RNA methyltransferase	-
U4959_RS14860 (U4959_14860)	mraY	3079833..3080951 (-)	1119	WP_004909640.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39018.71 Da
• Isoelectric Point: 4.5976

>NTDB_id=916900 U4959_RS14845 WP_004909635.1 3075145..3076203(-) (pilM) [Acinetobacter junii strain H1]
MLRLYRKPNKGLVGVDISSTTVKLLELSVKNGRYWVESYALMPLPENSVVEKSILNPEAVGDALERVVNLANPHTTNVAI
AVPTSMAINKIIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLADRLANPNRVNVLLVATRTENVDSRVEVLEL
AGLKAKIAEVESYAMERAFSVFADTLPMGANTVGILDIGHTMTTLSVMQKGKIIYTREQVFGGKQLTQDVQNRYGLSFEE
AGRAKKERSLPDDYDTEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=916900 U4959_RS14845 WP_004909635.1 3075145..3076203(-) (pilM) [Acinetobacter junii strain H1]
GTGCTCAGGTTATATCGTAAACCAAATAAGGGGTTAGTGGGTGTCGATATTAGTTCGACAACTGTTAAGTTATTGGAACT
CTCTGTTAAGAACGGTCGATATTGGGTTGAAAGTTATGCATTGATGCCACTACCTGAAAATAGTGTGGTTGAGAAAAGCA
TTTTAAACCCAGAAGCAGTTGGCGACGCGCTTGAACGAGTTGTTAATCTTGCAAACCCTCACACTACCAATGTGGCTATT
GCCGTGCCTACTTCGATGGCCATTAATAAAATCATTGAGATGGATGCTGACATGACGGATGATGAACGGGAAGTTCAAAT
TCGTATGGATGCAGAGCAATATATTCCATTCCCATTAGATGAAGTTAGTTTGGACTTTGAAGTCTTAGCTGATCGATTGG
CAAATCCTAATCGTGTCAATGTGTTATTAGTCGCTACGCGTACTGAGAATGTTGATTCACGTGTTGAAGTTCTGGAACTA
GCAGGTCTAAAGGCAAAGATTGCTGAAGTCGAAAGTTATGCGATGGAGCGTGCTTTTAGTGTATTCGCTGATACGCTACC
TATGGGTGCGAATACAGTGGGTATTCTAGATATTGGGCATACGATGACGACGTTATCGGTCATGCAAAAAGGTAAAATTA
TCTATACTCGTGAGCAAGTGTTTGGTGGAAAACAACTTACTCAAGACGTTCAAAATCGTTATGGTTTATCTTTTGAAGAA
GCTGGTCGAGCTAAAAAAGAAAGAAGTTTGCCAGATGATTACGATACCGAAGTTTTAGAACCATTTCTTGATGCAGTTGT
ACAACAAGCTGCACGTTCGCTGCAATTCTTTTTCTCATCATCTCAATTTAATGAGATTGACCACATCCTGTTAGCAGGTG
GTAATGCCAATATTCCAGGTCTAGCTAAACTGTTGCAACAAAAGCTAGGTTATCGAGTAACGATTGCCAACCCATTTTTA
CAAATGGGCTTTTCTCCCCAAATTGATATCAAAAAAATAGAAAATGATGCTTCATCGTTGATGGTGGCATGTGGATTAGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: S7Y3V7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	88.068	100	0.881
comM	Acinetobacter nosocomialis M2	87.784	100	0.878
comM	Acinetobacter baylyi ADP1	83.239	100	0.832
pilM	Legionella pneumophila strain ERS1305867	41.243	100	0.415