Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U8518_RS00300 Genome accession   NZ_CP141267
Coordinates   55376..56485 (-) Length   369 a.a.
NCBI ID   WP_101149750.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain MaAh001     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 50376..61485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U8518_RS00280 (U8518_00280) brnQ 50833..52140 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  U8518_RS00285 (U8518_00285) - 52332..53042 (-) 711 WP_101149753.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  U8518_RS00290 (U8518_00290) srmB 53171..54394 (+) 1224 WP_101149752.1 ATP-dependent RNA helicase SrmB -
  U8518_RS00295 (U8518_00295) yaaA 54571..55344 (-) 774 WP_101149751.1 peroxide stress protein YaaA -
  U8518_RS00300 (U8518_00300) pilU 55376..56485 (-) 1110 WP_101149750.1 type IVa pilus ATPase TapU Machinery gene
  U8518_RS00305 (U8518_00305) pilT 56514..57548 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  U8518_RS00310 (U8518_00310) - 57588..58289 (+) 702 WP_101149749.1 YggS family pyridoxal phosphate-dependent enzyme -
  U8518_RS00315 (U8518_00315) proC 58348..59172 (+) 825 WP_045789005.1 pyrroline-5-carboxylate reductase -
  U8518_RS00320 (U8518_00320) - 59213..59764 (+) 552 WP_016351969.1 YggT family protein -
  U8518_RS00325 (U8518_00325) yggU 59764..60063 (+) 300 WP_101149748.1 DUF167 family protein YggU -
  U8518_RS00330 (U8518_00330) - 60082..60501 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  U8518_RS00335 (U8518_00335) - 60558..60869 (-) 312 WP_101149747.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41053.20 Da        Isoelectric Point: 6.5035

>NTDB_id=916711 U8518_RS00300 WP_101149750.1 55376..56485(-) (pilU) [Aeromonas hydrophila strain MaAh001]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNMRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=916711 U8518_RS00300 WP_101149750.1 55376..56485(-) (pilU) [Aeromonas hydrophila strain MaAh001]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTATCTGCATACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTAGCCAAGCGCGGGCTGGTGCTGTTCGTCGGAGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGTAGCCTGATCACCCAGCGCGAGGTGGGTATAGACACAGCGTCATTCGACGT
GGCGCTGAAGAGTTCGCTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACATCGCCAGTTCTTGTTCGATCTCTCCTTCAACATGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
TTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

56.657

95.664

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.692

89.702

0.374
  pilT Legionella pneumophila strain Lp02

39.942

92.954

0.371
  pilT Legionella pneumophila strain ERS1305867

39.942

92.954

0.371