Detailed information NTG0916467

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: U5817_RS03500
• Genome accession: NZ_CP141259
• Coordinates: 791789..792997 (+)
• Length: 402 a.a.
• NCBI ID: WP_169130043.1
• Uniprot ID: -
• Organism: Aromatoleum evansii strain MAY27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 786789..797997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U5817_RS03485 (U5817_03475)	gmhB	788581..789120 (+)	540	WP_169130037.1	D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase	-
U5817_RS03490 (U5817_03480)	-	789129..789899 (+)	771	WP_169130039.1	lysophospholipid acyltransferase family protein	-
U5817_RS03495 (U5817_03485)	-	789953..791662 (+)	1710	WP_407279724.1	sensor histidine kinase	-
U5817_RS03500 (U5817_03490)	pilU	791789..792997 (+)	1209	WP_169130043.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
U5817_RS03505 (U5817_03495)	-	793427..793993 (-)	567	WP_407279725.1	phasin family protein	-
U5817_RS03510 (U5817_03500)	-	794323..795099 (-)	777	WP_169130049.1	enoyl-CoA hydratase	-
U5817_RS03515 (U5817_03505)	lptA	795129..795689 (-)	561	WP_169130051.1	lipopolysaccharide transport periplasmic protein LptA	-
U5817_RS03520 (U5817_03510)	lptC	795686..796267 (-)	582	WP_169130053.1	LPS export ABC transporter periplasmic protein LptC	-
U5817_RS03525 (U5817_03515)	-	796302..797297 (-)	996	WP_407279727.1	SIS domain-containing protein	-

Sequence

Protein

• Length: 402 a.a.
• Molecular weight: 44572.01 Da
• Isoelectric Point: 6.1951

>NTDB_id=916467 U5817_RS03500 WP_169130043.1 791789..792997(+) (pilU) [Aromatoleum evansii strain MAY27]
MSSALGIEEARSYMHRLLNAMHQMDGSDLFISVDFPPTVKAHGTMKALSQQRLTPDITRSLALSLMNDRQRAEFEAEMEC
NFAISIPGVCRFRVNVFVQQQHVGMVIRTIASEIPSLAKLGLPEVLKEIIMTKRGLVLVVGGTGSGKSTTLAAMIDHRNS
SGPGHIITVEDPVEYVHRNKQCLVTHREVGVDTHSWEHALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLCLGTLH
ANNANQTIDRIVNFFPEERRNQLLMDLSSNLRAIVSQRLIRTEDGKGRKAAIEILLNTPTIAEMIFKGQFQSIKEIMAKS
RELGMCTFDQALFDLYDAGFISYEEAIRNADSTNELRLQIKLKAERGQPKTSAAAELSLSIEEEKDEEEAEGSAAPGVKV
AG

Nucleotide

• Length: 1209 bp

>NTDB_id=916467 U5817_RS03500 WP_169130043.1 791789..792997(+) (pilU) [Aromatoleum evansii strain MAY27]
ATGTCGTCCGCCCTGGGCATAGAAGAAGCCCGTTCCTACATGCACAGGCTGCTCAATGCGATGCACCAGATGGACGGGTC
CGACCTGTTCATCTCGGTGGATTTTCCGCCGACCGTGAAGGCGCATGGCACGATGAAGGCGCTCAGCCAGCAGCGGCTCA
CGCCCGACATCACGCGTTCGCTGGCGCTTAGCCTCATGAATGACCGCCAGCGCGCAGAGTTCGAGGCAGAGATGGAGTGC
AATTTCGCCATCTCCATCCCCGGTGTGTGCCGCTTTCGCGTGAACGTCTTCGTCCAGCAGCAGCACGTCGGGATGGTGAT
CCGCACCATCGCGTCCGAGATTCCCTCGCTCGCCAAGCTAGGTCTGCCCGAGGTGCTCAAGGAGATCATCATGACCAAGC
GCGGCCTCGTGCTGGTCGTGGGCGGCACCGGTTCGGGAAAGTCGACGACGCTCGCGGCGATGATCGATCACCGCAACAGC
TCGGGGCCGGGACACATCATCACCGTCGAGGATCCGGTCGAGTACGTCCATCGCAACAAGCAGTGCCTCGTCACCCACCG
CGAGGTCGGCGTGGACACGCACTCCTGGGAGCACGCGTTGAAGAACACGCTGCGCCAGGCGCCCGATGTGATCCTGATCG
GCGAGATCCGCGACCGCGAGACGATGGAACACGCGATTGCCTTCGCCGAGACCGGCCACCTGTGCCTGGGCACGCTGCAT
GCGAACAACGCCAACCAGACGATAGACCGCATCGTCAATTTCTTCCCCGAGGAGCGCCGCAACCAGCTCCTGATGGATCT
GTCGTCGAACCTGCGGGCGATCGTGTCGCAGCGCCTGATCCGCACCGAAGACGGCAAGGGGCGCAAGGCCGCGATCGAGA
TCCTGCTCAACACCCCGACGATCGCGGAGATGATCTTCAAGGGGCAGTTCCAGTCCATCAAGGAGATCATGGCGAAGTCG
CGCGAGCTGGGCATGTGCACCTTCGACCAGGCCCTGTTCGACCTCTACGACGCCGGCTTCATCAGCTACGAGGAGGCCAT
CCGCAACGCCGACTCGACCAACGAGCTGCGCCTGCAGATCAAGCTGAAGGCCGAGCGCGGCCAGCCGAAGACCTCCGCGG
CCGCGGAATTGAGCCTGTCGATCGAGGAGGAGAAGGACGAGGAGGAGGCCGAGGGCTCCGCCGCTCCGGGCGTGAAGGTC
GCTGGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (105-282)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.946	92.04	0.515
pilU	Acinetobacter baylyi ADP1	55.085	88.06	0.485
pilU	Vibrio cholerae strain A1552	51.429	87.065	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.429	87.065	0.378
pilT	Legionella pneumophila strain Lp02	45.171	79.851	0.361
pilT	Legionella pneumophila strain ERS1305867	45.171	79.851	0.361