Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SPJ73_RS05590 Genome accession   NZ_CP140795
Coordinates   1207790..1209178 (-) Length   462 a.a.
NCBI ID   WP_150332209.1    Uniprot ID   -
Organism   Pasteurella multocida strain PMLO     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1202790..1214178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPJ73_RS05560 (SPJ73_05550) - 1202917..1204290 (-) 1374 WP_096743226.1 sodium:alanine symporter family protein -
  SPJ73_RS05565 (SPJ73_05555) - 1204760..1205008 (-) 249 WP_115299813.1 GNAT family N-acetyltransferase -
  SPJ73_RS05570 (SPJ73_05560) yacG 1205008..1205211 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  SPJ73_RS05575 (SPJ73_05565) coaE 1205222..1205842 (-) 621 WP_115299814.1 dephospho-CoA kinase -
  SPJ73_RS05580 (SPJ73_05570) - 1205854..1206579 (-) 726 WP_115299815.1 A24 family peptidase -
  SPJ73_RS05585 (SPJ73_05575) pilC 1206579..1207790 (-) 1212 WP_115299816.1 type II secretion system F family protein Machinery gene
  SPJ73_RS05590 (SPJ73_05580) pilB 1207790..1209178 (-) 1389 WP_150332209.1 GspE/PulE family protein Machinery gene
  SPJ73_RS05595 (SPJ73_05585) pilA 1209178..1209612 (-) 435 WP_150332210.1 prepilin peptidase-dependent pilin Machinery gene
  SPJ73_RS05600 (SPJ73_05590) ampD 1209772..1210338 (+) 567 WP_203331640.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SPJ73_RS05605 (SPJ73_05595) rppH 1210924..1211520 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  SPJ73_RS05610 (SPJ73_05600) - 1211523..1212317 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  SPJ73_RS05615 (SPJ73_05605) lgt 1212327..1213130 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  SPJ73_RS05620 (SPJ73_05610) - 1213136..1213987 (+) 852 WP_083004791.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52213.84 Da        Isoelectric Point: 6.3686

>NTDB_id=915624 SPJ73_RS05590 WP_150332209.1 1207790..1209178(-) (pilB) [Pasteurella multocida strain PMLO]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHFSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=915624 SPJ73_RS05590 WP_150332209.1 1207790..1209178(-) (pilB) [Pasteurella multocida strain PMLO]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATTTCAGTTTTGCTGAGTTGGGGATG
ACTGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.052

0.667
  pilB Haemophilus influenzae Rd KW20

67.329

98.052

0.66
  pilB Glaesserella parasuis strain SC1401

58.811

98.268

0.578
  pilB Acinetobacter baumannii D1279779

39.351

100

0.42
  pilB Vibrio parahaemolyticus RIMD 2210633

39.501

100

0.411
  pilB Vibrio campbellii strain DS40M4

39.004

100

0.407
  pilB Acinetobacter baylyi ADP1

37.675

100

0.407
  pilB Vibrio cholerae strain A1552

38.066

100

0.4
  pilB Legionella pneumophila strain ERS1305867

37.014

100

0.392