Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U1R67_RS07895 Genome accession   NZ_CP140118
Coordinates   1723424..1724533 (+) Length   369 a.a.
NCBI ID   WP_319855184.1    Uniprot ID   -
Organism   Aeromonas caviae strain A20-9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1718424..1729533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U1R67_RS07860 (U1R67_07865) - 1718460..1719290 (+) 831 WP_043153034.1 CPBP family intramembrane glutamic endopeptidase -
  U1R67_RS07865 (U1R67_07870) - 1719366..1719785 (-) 420 WP_202043103.1 DUF4426 domain-containing protein -
  U1R67_RS07870 (U1R67_07875) yggU 1719804..1720103 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  U1R67_RS07875 (U1R67_07880) - 1720103..1720654 (-) 552 WP_042014922.1 YggT family protein -
  U1R67_RS07880 (U1R67_07885) proC 1720678..1721502 (-) 825 WP_041210938.1 pyrroline-5-carboxylate reductase -
  U1R67_RS07885 (U1R67_07890) - 1721625..1722326 (-) 702 WP_049636471.1 YggS family pyridoxal phosphate-dependent enzyme -
  U1R67_RS07890 (U1R67_07895) pilT 1722366..1723400 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  U1R67_RS07895 (U1R67_07900) pilU 1723424..1724533 (+) 1110 WP_319855184.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U1R67_RS07900 (U1R67_07905) yaaA 1724566..1725339 (+) 774 WP_103857984.1 peroxide stress protein YaaA -
  U1R67_RS07905 (U1R67_07910) srmB 1725527..1726747 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  U1R67_RS07910 (U1R67_07915) - 1726875..1727585 (+) 711 WP_342041007.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  U1R67_RS07915 (U1R67_07920) brnQ 1727821..1729083 (+) 1263 WP_045523295.1 branched-chain amino acid transport system II carrier protein -
  U1R67_RS07920 (U1R67_07925) - 1729174..1729401 (-) 228 WP_128344179.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40853.00 Da        Isoelectric Point: 6.3155

>NTDB_id=912869 U1R67_RS07895 WP_319855184.1 1723424..1724533(+) (pilU) [Aeromonas caviae strain A20-9]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGAPGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFGLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=912869 U1R67_RS07895 WP_319855184.1 1723424..1724533(+) (pilU) [Aeromonas caviae strain A20-9]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCCCAGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTTGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAAGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGGCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.689

95.122

0.558
  pilU Acinetobacter baylyi ADP1

57.507

95.664

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377