Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   U0036_RS15510 Genome accession   NZ_CP139967
Coordinates   3329584..3331362 (+) Length   592 a.a.
NCBI ID   WP_063241558.1    Uniprot ID   A0A976GAI0
Organism   Cupriavidus oxalaticus strain HAMBI_2164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3324584..3336362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0036_RS15475 (U0036_15480) proB 3324757..3325875 (-) 1119 WP_063241562.1 glutamate 5-kinase -
  U0036_RS15480 (U0036_15485) obgE 3325925..3327022 (-) 1098 WP_063241561.1 GTPase ObgE -
  U0036_RS15485 (U0036_15490) rpmA 3327146..3327406 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  U0036_RS15490 (U0036_15495) rplU 3327446..3327757 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  U0036_RS15495 (U0036_15500) - 3327953..3328246 (-) 294 WP_063241560.1 hypothetical protein -
  U0036_RS15500 (U0036_15505) ispB 3328245..3329174 (+) 930 WP_063241559.1 octaprenyl diphosphate synthase -
  U0036_RS15510 (U0036_15515) pilF 3329584..3331362 (+) 1779 WP_063241558.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  U0036_RS15515 (U0036_15520) - 3331442..3332713 (+) 1272 WP_063241557.1 type II secretion system F family protein -
  U0036_RS15520 (U0036_15525) pilD 3332717..3333619 (+) 903 WP_063241556.1 prepilin peptidase Machinery gene
  U0036_RS15525 (U0036_15530) coaE 3333671..3334309 (+) 639 WP_063241555.1 dephospho-CoA kinase -
  U0036_RS15530 (U0036_15535) zapD 3334547..3335305 (+) 759 WP_063241554.1 cell division protein ZapD -
  U0036_RS15535 (U0036_15540) - 3335310..3335501 (+) 192 WP_029048086.1 DNA gyrase inhibitor YacG -
  U0036_RS15540 (U0036_15545) - 3335555..3335992 (-) 438 WP_063241553.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 65121.91 Da        Isoelectric Point: 7.7693

>NTDB_id=912072 U0036_RS15510 WP_063241558.1 3329584..3331362(+) (pilF) [Cupriavidus oxalaticus strain HAMBI_2164]
MTLGLALAQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLGLSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKYVRSAGEALGTLKNISPVQAERKMIEYDP
VAAAGAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRVRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDTKDNKDGKDGKDAKDPKDKDARETVEKAVDFRVSTLPTLFGEKIVMRILESSSDK
LDIDQLGYEPQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGL
TFAAALRSFLRQDPDVIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLA
RRLCTCKRPGELPRETLLDAGFREPDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQA
RKDGVLSLREAGLLKVRAGVTSLEEVLATTNT

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=912072 U0036_RS15510 WP_063241558.1 3329584..3331362(+) (pilF) [Cupriavidus oxalaticus strain HAMBI_2164]
ATGACACTCGGTCTTGCCCTGGCCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCGCAGCTGATCGATGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTGGCTCTGTTCGCGGCGGACA
AGTACCAGCTGCCGTTGCTGGACCTCAACCAGTACAACCTGACCAAGGTGCCGCCGGGGCTGGCGGGCAACCGCGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAAAACCGGTTGGTGCTGGGGCTGTCCGATCCGTCCAACCAGGCCGG
GCTGGACGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGTACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACGCTCAAGAACATCTCGCCGGTGCAGGCCGAGCGCAAGATGATCGAATACGACCCG
GTGGCGGCCGCCGGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGACGACGCCCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCATCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGGTCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCGCCGCTGGATATTCGCGACAAGATCGCCACCCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCGCGCTGCCCAAGGATACGAA
GGACAACAAGGACGGCAAGGACGGCAAGGACGCGAAGGATCCCAAGGACAAGGACGCCAGGGAAACCGTCGAGAAGGCCG
TCGATTTCCGCGTCTCGACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGTCCGACAAG
CTCGATATCGACCAGCTCGGCTACGAGCCGCAGCAGAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCGTACGGCATGGT
GCTGGTGACCGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCA
ATATCTCGACCGCCGAGGACCCGGCCGAAATCCAGCTGCCCGGCATCAACCAGGTCAATGTCAACGACAAGGCCGGCCTG
ACCTTTGCCGCGGCGCTGCGCTCGTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGCGAAATCCGCGACCTGGAGAC
CGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCACACCAACGACGCGCCCACCACGC
TGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCTCCAGCGTGCTGCTGATCACCGCGCAGCGGCTGGCG
CGGCGGCTGTGCACCTGCAAGCGCCCCGGCGAGCTGCCGCGCGAGACGCTGCTGGATGCGGGCTTCCGCGAACCTGATCT
GGACGGCAGCTGGCAGCCCTACCACCCGGTCGGGTGCGAGCGCTGCAACGGCAGCGGCTACAAGGGCCGCTGCGGCATCT
ACCAGGTCATGCCGATCACCGAGGCGATGCAGCAGATCATCCTGTCGCACGGCACCGCGCTGCAGATCGCCGAGCAGGCG
CGCAAGGACGGCGTGCTATCGTTGCGCGAGGCTGGGCTGCTCAAGGTCAGAGCGGGCGTCACGTCACTCGAAGAGGTGCT
GGCGACCACGAATACGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(288-482)

Type II/IV secretion system protein (T2SSE)

(187-260)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.333

100

0.51
  pilB Acinetobacter baumannii D1279779

49.831

100

0.498
  pilB Acinetobacter baylyi ADP1

50

95.946

0.48
  pilB Legionella pneumophila strain ERS1305867

48.506

96.115

0.466
  pilB Vibrio cholerae strain A1552

43.813

100

0.443
  pilB Vibrio campbellii strain DS40M4

45.081

94.426

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

45.063

94.088

0.424
  pilF Thermus thermophilus HB27

37.983

100

0.382
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.653

96.453

0.363