Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   U0449_RS09740 Genome accession   NZ_CP139862
Coordinates   1888207..1889055 (+) Length   282 a.a.
NCBI ID   WP_000705304.1    Uniprot ID   A0A0E7P1X9
Organism   Streptococcus pneumoniae strain 05H0020-2     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1883207..1894055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0449_RS09715 - 1883922..1884629 (+) 708 WP_000638502.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  U0449_RS09720 - 1884634..1885656 (+) 1023 WP_000609894.1 ribitol-5-phosphate dehydrogenase -
  U0449_RS09725 licA 1885673..1886542 (+) 870 WP_322397698.1 choline kinase LicA -
  U0449_RS09730 - 1886532..1887410 (+) 879 WP_000837609.1 DMT family transporter -
  U0449_RS09735 - 1887422..1888111 (+) 690 WP_000643970.1 sugar phosphate nucleotidyltransferase -
  U0449_RS09740 dprA/cilB/dalA 1888207..1889055 (+) 849 WP_000705304.1 DNA-processing protein DprA Machinery gene
  U0449_RS09745 - 1889939..1890982 (+) 1044 WP_001812710.1 TIGR00341 family protein -
  U0449_RS09750 topA 1891149..1893236 (+) 2088 WP_000179638.1 type I DNA topoisomerase -
  U0449_RS09755 - 1893457..1893816 (+) 360 WP_001220357.1 YbaN family protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31076.75 Da        Isoelectric Point: 5.4047

>NTDB_id=911528 U0449_RS09740 WP_000705304.1 1888207..1889055(+) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 05H0020-2]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=911528 U0449_RS09740 WP_000705304.1 1888207..1889055(+) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 05H0020-2]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E7P1X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae D39

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae R6

99.645

100

0.996
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

99.645

100

0.996
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.357

99.291

0.55
  dprA Staphylococcus aureus N315

37.456

100

0.376