Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   U0447_RS04465 Genome accession   NZ_CP139861
Coordinates   833845..834861 (-) Length   338 a.a.
NCBI ID   WP_075218762.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H2017-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 828845..839861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0447_RS04420 - 828922..829053 (-) 132 WP_000768904.1 hypothetical protein -
  U0447_RS04425 - 829054..830244 (-) 1191 WP_000167753.1 acetate kinase -
  U0447_RS04430 comYH 830295..831248 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  U0447_RS04435 - 831309..831903 (-) 595 Protein_840 class I SAM-dependent methyltransferase -
  U0447_RS04440 comGG/cglG 832040..832453 (-) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0447_RS04445 comGF/cglF 832431..832892 (-) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0447_RS04450 comGE/cglE 832855..833157 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0447_RS04455 comGD/cglD 833120..833524 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0447_RS04460 comGC/cglC 833517..833843 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  U0447_RS04465 comGB/cglB 833845..834861 (-) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0447_RS04470 comGA/cglA/cilD 834809..835750 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  U0447_RS04475 - 835826..836191 (-) 366 WP_000286415.1 DUF1033 family protein -
  U0447_RS04480 - 836342..837400 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  U0447_RS04485 nagA 837563..838714 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38478.51 Da        Isoelectric Point: 9.6073

>NTDB_id=911415 U0447_RS04465 WP_075218762.1 833845..834861(-) (comGB/cglB) [Streptococcus pneumoniae strain 16H2017-2]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=911415 U0447_RS04465 WP_075218762.1 833845..834861(-) (comGB/cglB) [Streptococcus pneumoniae strain 16H2017-2]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503