Detailed information NTG0911380

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: U0447_RS00750
• Genome accession: NZ_CP139861
• Coordinates: 144832..145680 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705304.1
• Uniprot ID: A0A0E7P1X9
• Organism: Streptococcus pneumoniae strain 16H2017-2
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 139832..150680

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0447_RS00735	-	140071..140430 (-)	360	WP_001237219.1	YbaN family protein	-
U0447_RS00740	topA	140651..142738 (-)	2088	WP_000179638.1	type I DNA topoisomerase	-
U0447_RS00745	-	142905..143948 (-)	1044	WP_001812710.1	TIGR00341 family protein	-
U0447_RS00750	dprA/cilB/dalA	144832..145680 (-)	849	WP_000705304.1	DNA-processing protein DprA	Machinery gene
U0447_RS00755	-	145777..146466 (-)	690	WP_000643970.1	sugar phosphate nucleotidyltransferase	-
U0447_RS00760	-	146478..147356 (-)	879	WP_000837618.1	DMT family transporter	-
U0447_RS00765	licA	147346..148215 (-)	870	WP_000411207.1	choline kinase LicA	-
U0447_RS00770	-	148232..149254 (-)	1023	WP_000609885.1	ribitol-5-phosphate dehydrogenase	-
U0447_RS00775	-	149259..149966 (-)	708	WP_000638502.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31076.75 Da
• Isoelectric Point: 5.4047

>NTDB_id=911380 U0447_RS00750 WP_000705304.1 144832..145680(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 16H2017-2]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=911380 U0447_RS00750 WP_000705304.1 144832..145680(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain 16H2017-2]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A0E7P1X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376