Detailed information NTG0909081

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NCY62_RS13385
• Genome accession: NZ_CP139356
• Coordinates: 2823744..2824862 (+)
• Length: 372 a.a.
• NCBI ID: WP_032055148.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain A45P
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2818744..2829862

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCY62_RS13375 (NCY62_13430)	yggS	2821868..2822560 (-)	693	WP_250733744.1	YggS family pyridoxal phosphate-dependent enzyme	-
NCY62_RS13380 (NCY62_13435)	pilT	2822687..2823724 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
NCY62_RS13385 (NCY62_13440)	pilU	2823744..2824862 (+)	1119	WP_032055148.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NCY62_RS13390 (NCY62_13445)	fur	2824932..2825369 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
NCY62_RS13395 (NCY62_13450)	omlA	2825481..2825879 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
NCY62_RS13400 (NCY62_13455)	-	2825944..2826258 (-)	315	WP_014205982.1	RnfH family protein	-
NCY62_RS13405 (NCY62_13460)	-	2826258..2827325 (-)	1068	WP_250733746.1	hypothetical protein	-
NCY62_RS13410 (NCY62_13465)	-	2827479..2827934 (-)	456	WP_002121698.1	bacteriohemerythrin	-
NCY62_RS13415 (NCY62_13470)	-	2828186..2829034 (-)	849	WP_057062207.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=909081 NCY62_RS13385 WP_032055148.1 2823744..2824862(+) (pilU) [Acinetobacter pittii strain A45P]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=909081 NCY62_RS13385 WP_032055148.1 2823744..2824862(+) (pilU) [Acinetobacter pittii strain A45P]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATCGGGCAACTATTACACTCCA
TCATGAGTGAAAAACAGCGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACTGATTCATTTGA
AGTAGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGATCGT
ATTATTCACTTCTTTGAAAGTGATCGCCACAGCCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	74.868	100	0.761
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363