Detailed information NTG0908464

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI73_RS10940
• Genome accession: NZ_CP139262
• Coordinates: 2289277..2290395 (+)
• Length: 372 a.a.
• NCBI ID: WP_002121759.1
• Uniprot ID: A0A022J839
• Organism: Acinetobacter pittii strain HUM1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2284277..2295395

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI73_RS10930 (SOI73_10930)	yggS	2287401..2288093 (-)	693	WP_002121742.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI73_RS10935 (SOI73_10935)	pilT	2288220..2289257 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI73_RS10940 (SOI73_10940)	pilU	2289277..2290395 (+)	1119	WP_002121759.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI73_RS10945 (SOI73_10945)	fur	2290465..2290902 (-)	438	WP_196257432.1	ferric iron uptake transcriptional regulator	-
SOI73_RS10950 (SOI73_10950)	omlA	2291014..2291412 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI73_RS10955 (SOI73_10955)	-	2291477..2291791 (-)	315	WP_002121714.1	RnfH family protein	-
SOI73_RS10960 (SOI73_10960)	-	2291791..2292858 (-)	1068	WP_005075306.1	hypothetical protein	-
SOI73_RS10965 (SOI73_10965)	-	2293012..2293467 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI73_RS10970 (SOI73_10970)	-	2293719..2294567 (-)	849	WP_002121748.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41945.18 Da
• Isoelectric Point: 6.6404

>NTDB_id=908464 SOI73_RS10940 WP_002121759.1 2289277..2290395(+) (pilU) [Acinetobacter pittii strain HUM1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908464 SOI73_RS10940 WP_002121759.1 2289277..2290395(+) (pilU) [Acinetobacter pittii strain HUM1]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A022J839

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363