Detailed information NTG0908435

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI70_RS12400
• Genome accession: NZ_CP139258
• Coordinates: 2585285..2586403 (+)
• Length: 372 a.a.
• NCBI ID: WP_002121759.1
• Uniprot ID: A0A022J839
• Organism: Acinetobacter pittii strain HUM3
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2580285..2591403

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI70_RS12390 (SOI70_12410)	yggS	2583409..2584101 (-)	693	WP_005075313.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI70_RS12395 (SOI70_12415)	pilT	2584228..2585265 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI70_RS12400 (SOI70_12420)	pilU	2585285..2586403 (+)	1119	WP_002121759.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI70_RS12405 (SOI70_12425)	fur	2586473..2586910 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SOI70_RS12410 (SOI70_12430)	omlA	2587022..2587420 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI70_RS12415 (SOI70_12435)	-	2587485..2587799 (-)	315	WP_002121714.1	RnfH family protein	-
SOI70_RS12420 (SOI70_12440)	-	2587799..2588866 (-)	1068	WP_005075306.1	hypothetical protein	-
SOI70_RS12425 (SOI70_12445)	-	2589020..2589475 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI70_RS12430 (SOI70_12450)	-	2589727..2590575 (-)	849	WP_002121748.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41945.18 Da
• Isoelectric Point: 6.6404

>NTDB_id=908435 SOI70_RS12400 WP_002121759.1 2585285..2586403(+) (pilU) [Acinetobacter pittii strain HUM3]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908435 SOI70_RS12400 WP_002121759.1 2585285..2586403(+) (pilU) [Acinetobacter pittii strain HUM3]
ATGGATTTTAACGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCCAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A022J839

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363