Detailed information NTG0908237

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SOI69_RS10845
• Genome accession: NZ_CP139248
• Coordinates: 2316626..2317744 (+)
• Length: 372 a.a.
• NCBI ID: WP_017398880.1
• Uniprot ID: A0A6H0FWV9
• Organism: Acinetobacter pittii strain HCG18
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2311626..2322744

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SOI69_RS10835 (SOI69_10835)	yggS	2314750..2315442 (-)	693	WP_044099923.1	YggS family pyridoxal phosphate-dependent enzyme	-
SOI69_RS10840 (SOI69_10840)	pilT	2315569..2316606 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
SOI69_RS10845 (SOI69_10845)	pilU	2316626..2317744 (+)	1119	WP_017398880.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SOI69_RS10850 (SOI69_10850)	fur	2317814..2318251 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
SOI69_RS10855 (SOI69_10855)	omlA	2318363..2318761 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
SOI69_RS10860 (SOI69_10860)	-	2318826..2319140 (-)	315	WP_320539317.1	RnfH family protein	-
SOI69_RS10865 (SOI69_10865)	-	2319140..2320207 (-)	1068	WP_320539318.1	hypothetical protein	-
SOI69_RS10870 (SOI69_10870)	-	2320361..2320816 (-)	456	WP_002121698.1	bacteriohemerythrin	-
SOI69_RS10875 (SOI69_10875)	-	2321068..2321916 (-)	849	WP_204086013.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6404

>NTDB_id=908237 SOI69_RS10845 WP_017398880.1 2316626..2317744(+) (pilU) [Acinetobacter pittii strain HCG18]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKSA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=908237 SOI69_RS10845 WP_017398880.1 2316626..2317744(+) (pilU) [Acinetobacter pittii strain HCG18]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGACAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGCGATAAAAGTGCG
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCAGGCATGGTATTACGGCGAATTGAAACCAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTACTAAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCCTTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTCGA
AATTGCCTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACGGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGC
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A6H0FWV9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363