Detailed information NTG0907371

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SFA35_RS16100
• Genome accession: NZ_CP139141
• Coordinates: 3597402..3598565 (-)
• Length: 387 a.a.
• NCBI ID: WP_320571539.1
• Uniprot ID: -
• Organism: Pseudomonas sp. HR96
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3592402..3603565

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SFA35_RS16080 (SFA35_16080)	-	3592886..3593953 (+)	1068	WP_320571535.1	microcin C ABC transporter permease YejB	-
SFA35_RS16085 (SFA35_16085)	-	3593953..3594987 (+)	1035	WP_320571536.1	ABC transporter permease	-
SFA35_RS16090 (SFA35_16090)	-	3595001..3596587 (+)	1587	WP_320571537.1	ABC transporter ATP-binding protein	-
SFA35_RS16095 (SFA35_16095)	-	3596618..3597388 (+)	771	WP_320571538.1	3-hydroxybutyrate dehydrogenase	-
SFA35_RS16100 (SFA35_16100)	pilU	3597402..3598565 (-)	1164	WP_320571539.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SFA35_RS16105 (SFA35_16105)	-	3598665..3601217 (+)	2553	WP_320571540.1	PAS domain-containing protein	-
SFA35_RS16110 (SFA35_16110)	-	3601214..3601489 (+)	276	WP_320571541.1	peptidylprolyl isomerase	-
SFA35_RS16115 (SFA35_16115)	-	3601560..3601754 (+)	195	WP_320571542.1	hypothetical protein	-
SFA35_RS16120 (SFA35_16120)	-	3601777..3602058 (-)	282	WP_320571543.1	DUF1652 domain-containing protein	-
SFA35_RS16125 (SFA35_16125)	-	3602216..3603199 (-)	984	WP_414058407.1	methyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 42581.63 Da
• Isoelectric Point: 6.2506

>NTDB_id=907371 SFA35_RS16100 WP_320571539.1 3597402..3598565(-) (pilU) [Pseudomonas sp. HR96]
MELQALLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSAEALKPGEVAAVAAGIMDAEQKVDFERDLEMNLALSVSGVGR
FRVNIFKQRNEVSIVARNIKLDIPRFEDLKLPPVLLDVIMQKRGLVLFVGATGSGKSTSLAALIDYRNRSSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFQAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRTQLLNDLGNNLQAFVSQRLVRTVDGKRRAAVEVMLGSPTVRDFIHRSEFSELKGLMEKSVNLGMQTFDQAL
YGLVVEGAIDEEEALKNADSVNNLRLRLKLHREGGVEQTTAPAAPVPAAEIASADWGLVSDRDEPHQ

Nucleotide

• Length: 1164 bp

>NTDB_id=907371 SFA35_RS16100 WP_320571539.1 3597402..3598565(-) (pilU) [Pseudomonas sp. HR96]
ATGGAACTCCAAGCCCTGCTCAAGATTTTGGCCACCCAGGATGGTTCGGACCTCTACCTGTCCACCGGGGCGCCGCCGTG
CGCCAAATTCAATGGCGTGCTCAAGCCGTTGAGTGCCGAAGCGCTCAAGCCAGGCGAGGTGGCCGCAGTGGCCGCCGGGA
TCATGGACGCCGAGCAGAAGGTCGATTTCGAACGTGACCTGGAGATGAACCTGGCCTTGTCGGTGTCCGGCGTCGGCCGG
TTTCGCGTGAACATTTTCAAGCAGCGCAACGAAGTGTCAATCGTCGCGCGCAACATCAAGCTGGACATCCCGCGCTTCGA
AGACCTCAAGCTGCCGCCGGTGCTGCTCGATGTGATCATGCAGAAACGCGGCCTGGTGCTGTTCGTCGGAGCCACCGGGT
CGGGCAAGTCGACCTCGCTGGCGGCGCTGATCGACTACCGCAACCGCAGCAGCAGTGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTCCAGGC
CGCGCTGAAGAATACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACGTTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCCGAAGATCGCCGCACGCAGCTGCTCAATGACTTGGGCAACAACCTGCAGGCCTTCGTCTCGCAGCG
CCTGGTACGCACCGTCGACGGCAAGCGCCGCGCAGCGGTGGAGGTGATGCTGGGCTCGCCGACTGTGCGCGATTTCATCC
ACCGCAGCGAGTTCAGCGAGCTGAAGGGGCTGATGGAGAAATCCGTCAACCTGGGCATGCAGACCTTCGACCAGGCGCTC
TACGGCCTGGTGGTGGAGGGTGCGATCGACGAGGAAGAGGCGCTGAAAAATGCCGACTCGGTGAACAACCTGCGCCTGCG
CCTCAAGCTGCACCGCGAAGGCGGCGTGGAGCAGACCACGGCACCGGCGGCGCCCGTGCCGGCGGCCGAGATCGCCAGCG
CCGATTGGGGGCTGGTCTCGGATCGGGACGAGCCGCACCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.182	93.54	0.535
pilU	Acinetobacter baylyi ADP1	52.101	92.248	0.481
pilU	Vibrio cholerae strain A1552	52.691	91.214	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.362	97.158	0.382
pilT	Pseudomonas stutzeri DSM 10701	40.988	88.889	0.364
pilT	Pseudomonas aeruginosa PAK	40.988	88.889	0.364