Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RZE78_RS21675 Genome accession   NZ_CP138515
Coordinates   4593220..4594953 (+) Length   577 a.a.
NCBI ID   WP_319427488.1    Uniprot ID   -
Organism   Variovorax paradoxus strain 2u118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4588220..4599953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE78_RS21640 (RZE78_21640) - 4588250..4588846 (+) 597 WP_319427483.1 CNP1-like family protein -
  RZE78_RS21645 (RZE78_21645) proB 4588881..4590020 (-) 1140 WP_319427485.1 glutamate 5-kinase -
  RZE78_RS21650 (RZE78_21650) cgtA 4590059..4591147 (-) 1089 WP_319427486.1 Obg family GTPase CgtA -
  RZE78_RS21655 (RZE78_21655) rpmA 4591218..4591475 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  RZE78_RS21660 (RZE78_21660) rplU 4591488..4591799 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  RZE78_RS21665 (RZE78_21665) - 4591988..4592980 (+) 993 WP_319427487.1 polyprenyl synthetase family protein -
  RZE78_RS21675 (RZE78_21675) pilB 4593220..4594953 (+) 1734 WP_319427488.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RZE78_RS21680 (RZE78_21680) pilC 4594983..4596212 (+) 1230 WP_319427489.1 type II secretion system F family protein Machinery gene
  RZE78_RS21685 (RZE78_21685) - 4596212..4597177 (+) 966 WP_319427490.1 prepilin peptidase -
  RZE78_RS21690 (RZE78_21690) coaE 4597188..4597793 (+) 606 WP_319427491.1 dephospho-CoA kinase -
  RZE78_RS21695 (RZE78_21695) - 4597873..4599366 (+) 1494 WP_319427492.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62712.98 Da        Isoelectric Point: 6.2073

>NTDB_id=903285 RZE78_RS21675 WP_319427488.1 4593220..4594953(+) (pilB) [Variovorax paradoxus strain 2u118]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAETI
NSIVGAEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASPPT
LIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHVCRAPADMPR
QALLDAGFKEEQLDGTWKPYRPVGCAACSNGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=903285 RZE78_RS21675 WP_319427488.1 4593220..4594953(+) (pilB) [Variovorax paradoxus strain 2u118]
ATGGCTGCTGCCGAACTTCCTGTTAAAGAAAACACGCAAATTGCCCTCCCGGGCCTTGCGCGTGCCTTGGTGTCGGCCGG
CAAGCTTCCTGCGAAGACCGCGGAAGACATCTATCAAAAGTCGCTGAGCGGCCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTTGCCCATACGCTTTCCAGCGCCTTCGGCGCGCCGCTGCTGGACCTGGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCACGCCTACCGGATCGTGGTTCTCAGCAAGCG
CAACAACCGTCTCATCGTTGCAACGGCCGACCCGTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGCATGATCGAAGCCGCGGCGGTCACCGCGGCAGAAACCATC
AACAGCATCGTCGGCGCCGAGTTCGAGTTCGACGACGTCACGGCCGACACCTCGGGCGATGCCAACGAACAGGCCATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTGGACGGCGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGCTTTCGCATCGACGGCGAACTGCGCGAGATCGCGAGCCCGCCCACG
CTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGGCTCGACATTTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTCATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTGGACCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTGGGCTACGACGCCGACGAAAAGGAGCGC
CTGCTGCATGCGATCGGCCGTCCCTACGGCATGGTGCTGGTGACGGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATCGCAACGGCGGAAGACCCTTCCGAAATCAACCTGCCCGGCG
TGAACCAGGTGAACGTCAACGAGCGCGCGGGACTCACCTTCGCCACGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGGAACATGGGCATTGCGCCGTTCAACATCGCCT
CCAGCGTGATCCTGATCACGGCGCAGCGGCTCGCGCGGCGCCTGTGCCACGTATGCCGCGCGCCGGCCGACATGCCGCGC
CAGGCGCTGCTCGACGCGGGCTTCAAGGAAGAACAGCTCGACGGTACCTGGAAGCCCTATCGGCCGGTCGGCTGCGCGGC
GTGCAGCAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGCCATCCAGGCGATCATCCTGC
GCGACGGCAGCGCACTGGACATCGCGCGCCAGTCCGAGGCCGAGGGCGTGCGCTCGCTGCGGCAGTCGGGCCTGCGAAAA
GTCATGCAAGGGCTCACCTCACTCGAAGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.521

98.44

0.527
  pilB Acinetobacter baylyi ADP1

53.476

97.227

0.52
  pilF Neisseria gonorrhoeae MS11

52.043

97.574

0.508
  pilB Legionella pneumophila strain ERS1305867

49.027

97.92

0.48
  pilB Vibrio cholerae strain A1552

46.536

97.574

0.454
  pilB Vibrio campbellii strain DS40M4

45.664

97.92

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

97.4

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.963

94.107

0.376
  pilF Thermus thermophilus HB27

37.478

98.96

0.371