Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RZE77_RS22470 Genome accession   NZ_CP138513
Coordinates   4804473..4806206 (+) Length   577 a.a.
NCBI ID   WP_319439340.1    Uniprot ID   -
Organism   Variovorax paradoxus strain SPNA7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4799473..4811206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE77_RS22435 (RZE77_22435) - 4799581..4800093 (+) 513 WP_319439335.1 CNP1-like family protein -
  RZE77_RS22440 (RZE77_22440) proB 4800128..4801267 (-) 1140 WP_319439336.1 glutamate 5-kinase -
  RZE77_RS22445 (RZE77_22445) cgtA 4801306..4802391 (-) 1086 WP_319439337.1 Obg family GTPase CgtA -
  RZE77_RS22450 (RZE77_22450) rpmA 4802462..4802719 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  RZE77_RS22455 (RZE77_22455) rplU 4802741..4803052 (-) 312 WP_319439338.1 50S ribosomal protein L21 -
  RZE77_RS22460 (RZE77_22460) - 4803241..4804233 (+) 993 WP_319439339.1 polyprenyl synthetase family protein -
  RZE77_RS22470 (RZE77_22470) pilB 4804473..4806206 (+) 1734 WP_319439340.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RZE77_RS22475 (RZE77_22475) pilC 4806236..4807465 (+) 1230 WP_319439341.1 type II secretion system F family protein Machinery gene
  RZE77_RS22480 (RZE77_22480) - 4807465..4808430 (+) 966 WP_319439342.1 prepilin peptidase -
  RZE77_RS22485 (RZE77_22485) coaE 4808441..4809046 (+) 606 WP_319427491.1 dephospho-CoA kinase -
  RZE77_RS22490 (RZE77_22490) - 4809125..4810618 (+) 1494 WP_319439343.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62655.92 Da        Isoelectric Point: 6.2073

>NTDB_id=903267 RZE77_RS22470 WP_319439340.1 4804473..4806206(+) (pilB) [Variovorax paradoxus strain SPNA7]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAETI
NSIVGAEFEFDDVTADTSGDASEQAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASPPT
LIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHVCRAPADMPR
QALLDAGFKEEQLDGTWKPYRPVGCAACSNGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=903267 RZE77_RS22470 WP_319439340.1 4804473..4806206(+) (pilB) [Variovorax paradoxus strain SPNA7]
ATGGCTGCTGCCGAACTTCCTGTTAAAGAAAACACGCAAATTGCCCTCCCGGGCCTTGCGCGTGCCTTGGTGTCGGCCGG
CAAGCTTCCTGCGAAGACCGCGGAAGACATCTATCAAAAGTCGCTGAGCGGCCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTTGCCCACACGCTTTCCAGTGCCTTCGGCGCGCCGCTGCTGGACCTGGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCACGCCTACCGGATCGTGGTTCTCAGCAAGCG
CAACAACCGTCTCATCGTTGCAACAGCCGATCCGTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGCATGATCGAAGCCGCCGCGGTCACCGCGGCGGAAACCATC
AACAGCATCGTCGGCGCCGAGTTCGAGTTCGACGACGTCACCGCGGACACCTCGGGCGATGCCAGCGAACAGGCCATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTGGACGGCGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGCGTGCGCTTTCGCATCGACGGCGAACTGCGCGAGATCGCGAGCCCGCCCACG
CTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGGCTCGACATTTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTGGACCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTGGGCTACGACGCCGACGAAAAGGAGCGC
CTGCTGCATGCGATCGGCCGTCCCTACGGCATGGTGCTGGTGACGGGCCCCACCGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATCGCAACGGCGGAAGACCCGTCCGAAATCAACCTGCCGGGCG
TGAACCAGGTGAACGTCAACGAGCGCGCCGGACTCACCTTCGCGGCGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGGAACATGGGCATTGCGCCGTTCAACATCGCCT
CCAGCGTGATCCTCATCACGGCGCAGCGGCTCGCGCGGCGCCTGTGCCACGTATGCCGCGCGCCAGCCGACATGCCGCGC
CAGGCGCTGCTCGACGCGGGCTTCAAGGAAGAACAGCTCGACGGTACCTGGAAGCCCTATCGGCCGGTCGGCTGCGCGGC
GTGCAGCAACGGCTACAAGGGGCGGGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGCCATCCAGGCGATCATCCTGC
GCGACGGCAGCGCACTGGACATCGCGCGCCAGTCCGAGGCCGAGGGCGTGCGCTCGCTGCGGCAGTCGGGCCTGCGAAAA
GTCATGCAAGGGCTCACCTCACTCGAAGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.697

98.44

0.529
  pilB Acinetobacter baylyi ADP1

53.654

97.227

0.522
  pilF Neisseria gonorrhoeae MS11

52.043

97.574

0.508
  pilB Legionella pneumophila strain ERS1305867

49.027

97.92

0.48
  pilB Vibrio cholerae strain A1552

46.359

97.574

0.452
  pilB Vibrio campbellii strain DS40M4

45.664

97.92

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

97.4

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.963

94.107

0.376
  pilF Thermus thermophilus HB27

37.478

98.96

0.371