Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RZE79_RS06480 Genome accession   NZ_CP138496
Coordinates   1440023..1441756 (-) Length   577 a.a.
NCBI ID   WP_319359496.1    Uniprot ID   -
Organism   Variovorax sp. EBFNA2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1435023..1446756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZE79_RS06460 (RZE79_06460) - 1435609..1437102 (-) 1494 WP_319359492.1 MBOAT family protein -
  RZE79_RS06465 (RZE79_06465) coaE 1437179..1437790 (-) 612 WP_319359493.1 dephospho-CoA kinase -
  RZE79_RS06470 (RZE79_06470) - 1437804..1438769 (-) 966 WP_319359494.1 A24 family peptidase -
  RZE79_RS06475 (RZE79_06475) pilC 1438769..1439998 (-) 1230 WP_319359495.1 type II secretion system F family protein Machinery gene
  RZE79_RS06480 (RZE79_06480) pilB 1440023..1441756 (-) 1734 WP_319359496.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RZE79_RS06490 (RZE79_06490) - 1441999..1442991 (-) 993 WP_153286198.1 polyprenyl synthetase family protein -
  RZE79_RS06495 (RZE79_06495) rplU 1443174..1443485 (+) 312 WP_042578903.1 50S ribosomal protein L21 -
  RZE79_RS06500 (RZE79_06500) rpmA 1443498..1443758 (+) 261 WP_013543198.1 50S ribosomal protein L27 -
  RZE79_RS06505 (RZE79_06505) cgtA 1443832..1444908 (+) 1077 WP_192193331.1 Obg family GTPase CgtA -
  RZE79_RS06510 (RZE79_06510) proB 1444989..1446128 (+) 1140 WP_319359497.1 glutamate 5-kinase -
  RZE79_RS06515 (RZE79_06515) - 1446163..1446756 (-) 594 WP_319359498.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62552.87 Da        Isoelectric Point: 5.8801

>NTDB_id=903157 RZE79_RS06480 WP_319359496.1 1440023..1441756(-) (pilB) [Variovorax sp. EBFNA2]
MAAAEIPVKETTQIALPGLARALVSAGKLAAKSAEEIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAASAAETI
NSIVGTEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMLCRVPIDIPR
EALLEAGFNEADINGTWKPYRPVGCAACSGGYKGRVGIYQVMPITEAIQEIILRDGSALDIANQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=903157 RZE79_RS06480 WP_319359496.1 1440023..1441756(-) (pilB) [Variovorax sp. EBFNA2]
ATGGCCGCTGCCGAAATTCCCGTTAAAGAAACTACGCAGATCGCCCTTCCCGGCTTGGCGCGCGCTTTGGTGTCCGCCGG
CAAGCTTGCAGCGAAATCAGCCGAAGAGATCTATCAGAAGTCGCTGAGCGGCCGCACCAGCTTCATCGCCGAACTGACCG
GCACCGGCGCCGTCTCTGCTGCAGACCTCGCCCACACCCTGTCGAGCGCCTTCGGCGCGCCACTGCTCGACCTCGATGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTCTGCCACGCCTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCCACGGCCGACCCGTCGGACCAGCAGGCCGCGGAGAAGATCAAGTTCGCCTCGCAGATGG
GCGTCGACTGGGTCATCGCGGAGTACGACAAGCTCTCGCGCATGATCGAAGCCGCCGCAGCCAGTGCGGCTGAAACGATC
AACAGCATCGTCGGCACCGAGTTCGAGTTCGACGATGTCACGGCCGACACGTCGGGCGACGCCAACGAGCAGGCCATCGC
CGAAGTCGAAGACGCCCCCGTGGTGCGCTTCCTGCACAAGATGCTGCTCGACGCAGTGAGCATGCGGGCTTCGGACATTC
ACTTCGAGCCCTACGAACACCACTATCGGGTGCGCTTCCGGGTCGATGGCGAGTTGCGCGAAATCGCCTCCCCGCCCACG
GTCATCAAGGACAAGCTCGCCTCGCGCATCAAGGTCATTTCCCGGCTCGACATCTCGGAAAAACGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTCGTGCGTATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCCCTGGGCTACGACGCCGACGAGAAAGAACGG
CTCCTCCACGCCATCGGCCGCCCGTACGGCATGGTGCTGGTGACCGGCCCCACGGGCTCGGGCAAGACCGTGTCGCTGTA
CACCTGCCTGAACCTGCTCAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGATCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTCACCTTCGCTGCGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACCGGCCACTTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTGACGCGCATGCGCAACATGGGCATCGCGCCCTTCAACATTGCGT
CGAGCGTGATCCTGATCACTGCCCAACGCCTTGCGCGCCGGCTGTGCATGCTGTGTCGCGTGCCGATCGACATCCCGCGC
GAAGCCCTGCTTGAAGCCGGCTTCAACGAGGCAGACATCAACGGCACCTGGAAGCCGTACCGCCCGGTCGGCTGCGCCGC
CTGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATTACGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGTGCGCTGGACATCGCGAACCAATCCGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCCGGACTTCGCAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTCGTGGCCTGCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-157)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.484

99.48

0.532
  pilB Acinetobacter baumannii D1279779

54.286

97.054

0.527
  pilF Neisseria gonorrhoeae MS11

52.669

97.4

0.513
  pilB Legionella pneumophila strain ERS1305867

49.027

97.92

0.48
  pilB Vibrio cholerae strain A1552

46.429

97.054

0.451
  pilB Vibrio campbellii strain DS40M4

45.826

97.574

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

97.227

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.254

97.574

0.383
  pilF Thermus thermophilus HB27

38.313

98.614

0.378