Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CTP10_RS14530 Genome accession   NZ_AP025170
Coordinates   3155702..3157462 (+) Length   586 a.a.
NCBI ID   WP_116318215.1    Uniprot ID   A0A388RGF5
Organism   Cupriavidus sp. P-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3150702..3162462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTP10_RS14495 (CTP10_R29000) proB 3150852..3151970 (-) 1119 WP_116318211.1 glutamate 5-kinase -
  CTP10_RS14500 (CTP10_R29010) obgE 3152016..3153113 (-) 1098 WP_116318212.1 GTPase ObgE -
  CTP10_RS14505 (CTP10_R29020) rpmA 3153275..3153535 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  CTP10_RS14510 (CTP10_R29030) rplU 3153575..3153886 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  CTP10_RS14515 (CTP10_R29040) - 3154083..3154313 (-) 231 WP_147316191.1 hypothetical protein -
  CTP10_RS14520 (CTP10_R29050) ispB 3154363..3155292 (+) 930 WP_116318214.1 octaprenyl diphosphate synthase -
  CTP10_RS14530 (CTP10_R29070) pilF 3155702..3157462 (+) 1761 WP_116318215.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CTP10_RS14535 (CTP10_R29080) - 3157542..3158813 (+) 1272 WP_116318216.1 type II secretion system F family protein -
  CTP10_RS14540 (CTP10_R29090) - 3158817..3159719 (+) 903 WP_116318217.1 A24 family peptidase -
  CTP10_RS14545 (CTP10_R29100) coaE 3159771..3160409 (+) 639 WP_116318218.1 dephospho-CoA kinase -
  CTP10_RS14550 (CTP10_R29110) zapD 3160647..3161405 (+) 759 WP_116318219.1 cell division protein ZapD -
  CTP10_RS14555 (CTP10_R29120) - 3161423..3161614 (+) 192 WP_116318220.1 DNA gyrase inhibitor YacG -
  CTP10_RS14560 (CTP10_R29140) - 3161744..3161944 (-) 201 Protein_2855 hypothetical protein -
  CTP10_RS14565 (CTP10_R29150) - 3161958..3162395 (-) 438 WP_116318221.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 586 a.a.        Molecular weight: 64770.54 Da        Isoelectric Point: 8.0661

>NTDB_id=90255 CTP10_RS14530 WP_116318215.1 3155702..3157462(+) (pilF) [Cupriavidus sp. P-10]
MTLGLALTQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQTERKMIEYDP
VAAASAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDNKDSKDAKDSKDKDVKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDKLDIDQL
GYEPQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAAL
RSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCTC
KRPGELPRETLRDAGFREQDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQARKDGVL
SLREAGLLKVRQGVTSLEEVLATTNT

Nucleotide


Download         Length: 1761 bp        

>NTDB_id=90255 CTP10_RS14530 WP_116318215.1 3155702..3157462(+) (pilF) [Cupriavidus sp. P-10]
ATGACACTCGGTCTTGCCCTGACCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCCCAGTTGATCGACGAGATCGTCGGCAGCGGCACCATGAGCGCGCACGACCTCGCGCTGTTCGCGGCGGACA
AATACCAGCTGCCGCTGCTGGACCTGAACCAGTACAACCTGACCAAGGTGCCGCCGGGCCTGGCGGGCAACCGGGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCGATGTCCGATCCGTCCAACCAGGCCGG
GCTGGACGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACACTGAAGAACATCTCGCCGGTGCAGACCGAGCGCAAGATGATCGAGTACGACCCG
GTGGCGGCCGCCAGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGATGACGCCCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCGTCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGATCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCGCCGCTGGATATCCGCGACAAGATCGCCACGCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGGCGCATGAAGCTGCTGATCGCGCTGCCCAAGGACAACAA
GGACAGCAAGGACGCGAAGGATTCCAAGGACAAGGACGTCAAGGAAACGGTCGAGAAGGCCGTCGATTTCCGCGTCTCGA
CGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGAGCGACAAGCTCGACATCGACCAGCTC
GGCTACGAACCGCAGCAGAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCGTACGGCATGGTACTGGTGACAGGCCCCAC
CGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCAATATCTCGACCGCCGAGG
ATCCGGCTGAAATCCAGCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAGGCCGGCCTCACCTTCGCCGCGGCGCTG
CGCTCGTTCCTGCGGCAGGATCCGGACATCATCATGGTCGGCGAAATCCGTGACCTGGAAACCGCCGATATCTCGATCAA
GGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCACACCAACGACGCGCCCACCACGCTGACGCGGCTGATGAACA
TGGGCGTGGCGCCGTTCAACATCGCCTCCAGCGTGCTGATGATCACCGCGCAGCGGCTGGCGCGGCGGCTGTGCACCTGC
AAGCGCCCCGGCGAGCTGCCGCGCGAGACGCTGCGGGATGCGGGCTTCCGCGAACAGGACCTGGACGGCAGCTGGCAGCC
CTACCACCCGGTCGGTTGCGAGCGCTGCAACGGCAGCGGCTACAAAGGCCGCTGCGGCATTTACCAGGTCATGCCGATCA
CCGAGGCGATGCAGCAGATCATCCTGTCGCACGGCACCGCCCTGCAGATCGCCGAGCAGGCGCGCAAGGACGGCGTGCTA
TCGTTGCGCGAGGCTGGGCTGCTGAAGGTCAGGCAGGGCGTCACGTCACTCGAAGAGGTGCTGGCGACCACGAATACGTA
G

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(279-476)

Type II/IV secretion system protein (T2SSE)

(187-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A388RGF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.761

100

0.512
  pilB Acinetobacter baumannii D1279779

50.947

99.147

0.505
  pilB Acinetobacter baylyi ADP1

50.712

95.904

0.486
  pilB Legionella pneumophila strain ERS1305867

49.378

96.075

0.474
  pilB Vibrio cholerae strain A1552

44.426

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

44.964

94.881

0.427
  pilB Vibrio campbellii strain DS40M4

45.027

94.369

0.425
  pilF Thermus thermophilus HB27

38.879

97.44

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.811

97.611

0.379

Multiple sequence alignment