Detailed information NTG0902523

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SD196_RS01905
• Genome accession: NZ_CP138377
• Coordinates: 409526..410671 (+)
• Length: 381 a.a.
• NCBI ID: WP_331315650.1
• Uniprot ID: -
• Organism: Pseudomonas sp. W03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 404526..415671

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SD196_RS01880 (SD196_01885)	-	405733..406023 (-)	291	WP_275609791.1	DUF167 domain-containing protein	-
SD196_RS01885 (SD196_01890)	-	406029..406622 (-)	594	WP_275609792.1	YggT family protein	-
SD196_RS01890 (SD196_01895)	proC	406636..407469 (-)	834	WP_343578259.1	pyrroline-5-carboxylate reductase	-
SD196_RS01895 (SD196_01900)	-	407487..408176 (-)	690	WP_275609794.1	YggS family pyridoxal phosphate-dependent enzyme	-
SD196_RS01900 (SD196_01905)	pilT	408250..409284 (+)	1035	WP_151189058.1	type IV pilus twitching motility protein PilT	Machinery gene
SD196_RS01905 (SD196_01910)	pilU	409526..410671 (+)	1146	WP_331315650.1	type IV pilus ATPase PilU	Machinery gene
SD196_RS01910 (SD196_01915)	-	410764..411168 (+)	405	WP_275609796.1	NINE protein	-
SD196_RS01915 (SD196_01920)	-	411392..412765 (+)	1374	WP_331315653.1	cystathionine beta-synthase	-
SD196_RS01920 (SD196_01925)	-	412762..413931 (+)	1170	WP_343578260.1	PLP-dependent aspartate aminotransferase family protein	-
SD196_RS01925 (SD196_01930)	-	414145..415416 (-)	1272	WP_275609799.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42574.87 Da
• Isoelectric Point: 6.7259

>NTDB_id=902523 SD196_RS01905 WP_331315650.1 409526..410671(+) (pilU) [Pseudomonas sp. W03]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTFEDLKLPEVLKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=902523 SD196_RS01905 WP_331315650.1 409526..410671(+) (pilU) [Pseudomonas sp. W03]
ATGGAATTCGAGAAGCTGTTGCGCCTCATGGTGGAAAAGGGTGCCTCCGACCTCTTCATCACGGCGGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTCTCGG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTTGGCCGT
TTCCGCGTCAGCGCCTTTTATCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCTTCGA
GGATCTGAAGCTTCCCGAAGTCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATTGGCTACCGCAACAAGAACTCCACCGGCCACATTATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGATTCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CGGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTACCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAAGACGCGCTGGCCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACGCCGACCACCTCTCCACCATGACCCAGGGGCTGACCCTGGAAATGAGCGACG
ACGACCCCAACCGCCGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	89.239	100	0.892
pilU	Acinetobacter baylyi ADP1	63.83	98.688	0.63
pilU	Vibrio cholerae strain A1552	59.569	97.375	0.58
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.317	87.664	0.362
pilT	Vibrio cholerae strain A1552	41.317	87.664	0.362