Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SC043_RS03705 Genome accession   NZ_CP138309
Coordinates   698819..699466 (+) Length   215 a.a.
NCBI ID   WP_010906131.1    Uniprot ID   A0A3N6KQH1
Organism   Lactococcus lactis strain B26     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 693819..704466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SC043_RS03680 (SC043_03680) - 693948..695126 (+) 1179 WP_021214596.1 SLC13 family permease -
  SC043_RS03685 (SC043_03685) - 695299..695862 (+) 564 WP_004255233.1 GNAT family N-acetyltransferase -
  SC043_RS03690 (SC043_03690) - 695966..696364 (+) 399 WP_004255236.1 hypothetical protein -
  SC043_RS03695 (SC043_03695) - 696469..697401 (+) 933 WP_004255238.1 ABC transporter ATP-binding protein -
  SC043_RS03700 (SC043_03700) - 697398..698759 (+) 1362 WP_021214597.1 ABC transporter permease -
  SC043_RS03705 (SC043_03705) comEA 698819..699466 (+) 648 WP_010906131.1 ComEA family DNA-binding protein Machinery gene
  SC043_RS03710 (SC043_03710) comEC 699447..701657 (+) 2211 WP_010906130.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SC043_RS03715 (SC043_03715) - 701942..702718 (+) 777 WP_004255244.1 alpha/beta hydrolase family protein -
  SC043_RS03720 (SC043_03720) - 702902..703117 (+) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  SC043_RS03725 (SC043_03725) atpB 703162..703875 (+) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  SC043_RS03730 (SC043_03730) atpF 703890..704396 (+) 507 WP_010906128.1 F0F1 ATP synthase subunit B -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23624.76 Da        Isoelectric Point: 4.8659

>NTDB_id=901698 SC043_RS03705 WP_010906131.1 698819..699466(+) (comEA) [Lactococcus lactis strain B26]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=901698 SC043_RS03705 WP_010906131.1 698819..699466(+) (comEA) [Lactococcus lactis strain B26]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3N6KQH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

76.147

100

0.772
  comEA/celA/cilE Streptococcus mitis SK321

44.907

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.578

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae D39

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae R6

41.553

100

0.423
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.743

100

0.423
  comEA Latilactobacillus sakei subsp. sakei 23K

36.564

100

0.386