Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LDN97_RS02920 Genome accession   NZ_AP024940
Coordinates   572313..573422 (-) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas caviae strain KAM339     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 567313..578422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN97_RS02895 (KAM339_005470) - 567446..567673 (+) 228 WP_223956168.1 hypothetical protein -
  LDN97_RS02900 (KAM339_005480) brnQ 567763..569070 (-) 1308 WP_223956169.1 branched-chain amino acid transport system II carrier protein -
  LDN97_RS02905 (KAM339_005490) - 569261..569971 (-) 711 WP_223956170.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  LDN97_RS02910 (KAM339_005500) srmB 570099..571319 (+) 1221 WP_201888037.1 ATP-dependent RNA helicase SrmB -
  LDN97_RS02915 (KAM339_005510) yaaA 571507..572280 (-) 774 WP_042014926.1 peroxide stress protein YaaA -
  LDN97_RS02920 (KAM339_005520) pilU 572313..573422 (-) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDN97_RS02925 (KAM339_005530) pilT 573446..574480 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  LDN97_RS02930 (KAM339_005540) - 574520..575221 (+) 702 WP_223956171.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDN97_RS02935 (KAM339_005550) proC 575345..576169 (+) 825 WP_223956172.1 pyrroline-5-carboxylate reductase -
  LDN97_RS02940 (KAM339_005560) - 576193..576744 (+) 552 WP_010673053.1 YggT family protein -
  LDN97_RS02945 (KAM339_005570) yggU 576744..577043 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  LDN97_RS02950 (KAM339_005580) - 577062..577481 (+) 420 WP_029313762.1 DUF4426 domain-containing protein -
  LDN97_RS02955 (KAM339_005590) - 577557..578387 (-) 831 WP_223956173.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=89579 LDN97_RS02920 WP_042882153.1 572313..573422(-) (pilU) [Aeromonas caviae strain KAM339]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=89579 LDN97_RS02920 WP_042882153.1 572313..573422(-) (pilU) [Aeromonas caviae strain KAM339]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTTTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment