Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CYJ98_RS07530 Genome accession   NZ_CP136962
Coordinates   1631774..1633453 (+) Length   559 a.a.
NCBI ID   WP_101756015.1    Uniprot ID   A0A9X7F759
Organism   Neisseria perflava strain UMB0023     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1626774..1638453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYJ98_RS07515 (CYJ98_007515) - 1627016..1627273 (+) 258 WP_016687622.1 SemiSWEET family transporter -
  CYJ98_RS07520 (CYJ98_007520) - 1627560..1628132 (+) 573 WP_101756013.1 DUF1643 domain-containing protein -
  CYJ98_RS07525 (CYJ98_007525) carB 1628156..1631371 (+) 3216 WP_101756014.1 carbamoyl-phosphate synthase large subunit -
  CYJ98_RS07530 (CYJ98_007530) pilF 1631774..1633453 (+) 1680 WP_101756015.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CYJ98_RS07535 (CYJ98_007535) pilG 1633533..1634774 (+) 1242 WP_101756016.1 type II secretion system F family protein Machinery gene
  CYJ98_RS07540 (CYJ98_007540) pilD 1634776..1635663 (+) 888 WP_101756017.1 prepilin peptidase Machinery gene
  CYJ98_RS07545 (CYJ98_007545) coaE 1635663..1636283 (+) 621 WP_101756018.1 dephospho-CoA kinase -
  CYJ98_RS07550 (CYJ98_007550) yacG 1636280..1636462 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  CYJ98_RS07555 (CYJ98_007555) glyQ 1636544..1637443 (+) 900 WP_101756019.1 glycine--tRNA ligase subunit alpha -
  CYJ98_RS07560 (CYJ98_007560) lspA 1637592..1638098 (+) 507 WP_167382871.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62266.49 Da        Isoelectric Point: 6.0382

>NTDB_id=893794 CYJ98_RS07530 WP_101756015.1 1631774..1633453(+) (pilF) [Neisseria perflava strain UMB0023]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSSKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=893794 CYJ98_RS07530 WP_101756015.1 1631774..1633453(+) (pilF) [Neisseria perflava strain UMB0023]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGAGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTAGTCGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTCGTAGTTCCCGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGTCAACGTTCAACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACGCAGCAATCGCAATCG
CTTTATATCGACAATGAAGAGGCTGAAGACGGCCCTATCCCTCGTTTCATCCACAAAACCCTATCCGATGCCCTGAACGC
GGGCGCATCAGACATCCACTTTGAATTCTACGAACAAATGGCGCGCGTCCGTTTCCGTGTGGACGGACAGTTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTAATGGCGCGTTTGGATATTTCCGAAAAA
CGCATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACTTTGCC
GACATTGTTTGGTGAAAAAGTAGTGATGCGTATCCTAAATTCCGATGGCACATCACTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATTAACCGCCCTTACGGCATGGTATTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCGCTCTATACCTGCCTGAGCATTTTGAATACGGAAGATGTGAACATTTCGACGGCAGAAGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGTCTGACGTTTGCCGCCGCACTCCGATCCT
TCTTACGTCAAGATCCTGATATTATTATGGTCGGTGAGATTCGTGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
CGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTAATTATGGCGCAAAGGTTGTTGCGCAGACTGTGCCCGAACTGCAAAC
GTGAAGTTGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGTGCTGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.281

100

0.773
  pilB Acinetobacter baylyi ADP1

51.053

100

0.521
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

47.173

100

0.478
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio campbellii strain DS40M4

44.306

100

0.445
  pilF Thermus thermophilus HB27

38.941

94.633

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.122

93.739

0.367