Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R1Z03_RS02310 Genome accession   NZ_CP136797
Coordinates   478253..479983 (-) Length   576 a.a.
NCBI ID   WP_284405479.1    Uniprot ID   -
Organism   Acidovorax sp. BLS4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473253..484983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1Z03_RS02280 (R1Z03_02280) - 473928..474383 (+) 456 WP_317356515.1 NUDIX domain-containing protein -
  R1Z03_RS02285 (R1Z03_02285) - 474424..474672 (-) 249 WP_317356518.1 DNA gyrase inhibitor YacG -
  R1Z03_RS02290 (R1Z03_02290) zapD 474686..475441 (-) 756 WP_272565520.1 cell division protein ZapD -
  R1Z03_RS02295 (R1Z03_02295) coaE 475506..476126 (-) 621 WP_317356523.1 dephospho-CoA kinase -
  R1Z03_RS02300 (R1Z03_02300) - 476131..477015 (-) 885 WP_317356525.1 A24 family peptidase -
  R1Z03_RS02305 (R1Z03_02305) pilC 477015..478232 (-) 1218 WP_272565514.1 type II secretion system F family protein Machinery gene
  R1Z03_RS02310 (R1Z03_02310) pilB 478253..479983 (-) 1731 WP_284405479.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R1Z03_RS02320 (R1Z03_02320) - 480194..481123 (-) 930 WP_317360211.1 polyprenyl synthetase family protein -
  R1Z03_RS02325 (R1Z03_02325) rplU 481380..481691 (+) 312 WP_317356529.1 50S ribosomal protein L21 -
  R1Z03_RS02330 (R1Z03_02330) rpmA 481706..481963 (+) 258 WP_272548923.1 50S ribosomal protein L27 -
  R1Z03_RS02335 (R1Z03_02335) cgtA 482087..483157 (+) 1071 WP_317356534.1 Obg family GTPase CgtA -
  R1Z03_RS02340 (R1Z03_02340) proB 483239..484378 (+) 1140 WP_284429253.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62722.19 Da        Isoelectric Point: 5.7591

>NTDB_id=892660 R1Z03_RS02310 WP_284405479.1 478253..479983(-) (pilB) [Acidovorax sp. BLS4]
MAVDTAPKEGAAIALPGLGRALMSAGKLSQKSAEDIFRKSQVGKTSFIAELTGSGAVSSADLAHTVSAVFGAPLLDLEAI
DPLRIPKDLLDPKICLAYRVVALSKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEYDKLIKLIEANTKSVSESME
SLSGGGDFEFDDVKVEEAPENSDSANDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIASPPIA
IKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKERL
MHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKSFLRQDPDI
IMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPVCKAPADIPHE
TLLEAGYKEDEIDGTWVTYRPVGCSACNNGYKGRLGIYQVMPISEEIQRIILRDGSALEIAKQAKAEGVRSLREAGLHKA
KMGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=892660 R1Z03_RS02310 WP_284405479.1 478253..479983(-) (pilB) [Acidovorax sp. BLS4]
ATGGCCGTTGATACCGCACCCAAAGAAGGCGCCGCAATTGCCCTGCCAGGTCTAGGCCGGGCATTGATGTCAGCCGGCAA
GCTGAGTCAAAAATCAGCCGAAGATATCTTTCGCAAGTCACAGGTTGGCAAAACAAGCTTCATTGCCGAGTTGACCGGGT
CGGGGGCTGTTTCCTCTGCCGATCTGGCGCACACGGTTTCTGCCGTATTCGGTGCGCCCTTGCTGGATCTGGAAGCCATC
GATCCCCTTCGGATTCCCAAAGATCTTCTGGATCCGAAGATCTGCCTCGCCTATCGTGTCGTGGCACTGAGCAAGCGCAA
CAACCGGCTGATCGTGGCCACCGCAGACCCCACCGACCAGGAGGCAGCGGAAAAGATCAAATTCACCACCCAGATGGGGG
TGGACTGGATCATTGCGGAATACGACAAGCTCATCAAGCTGATCGAGGCCAATACCAAGTCGGTCAGCGAGTCGATGGAA
AGCCTGTCGGGCGGGGGAGATTTCGAGTTCGACGATGTCAAGGTCGAAGAAGCTCCCGAAAACTCCGACAGCGCCAACGA
CGTTGAAGACGCGCCGATCGTCAAGTTCCTTCACAAAATGCTTTTGGACGCGTTCAACATGCGCGCTTCCGATTTGCATT
TCGAACCTTACGAGCACCAGTACCGGGTGCGTTTTCGCATCGATGGCGAATTGCGCGAAATTGCTTCGCCCCCCATTGCC
ATCAAGGACAAGCTGGCGTCCCGAATCAAGGTCATTTCCAGACTGGACATTTCGGAAAAGCGTGTCCCACAAGATGGCCG
CATGAAGCTCAAAGTCGGCCCGGACCGGGTCATCGACTTTCGGGTGAGCACGCTGCCTACGCTCTTTGGCGAAAAGATCG
TGATCCGGATTTTGGATCCCAGCAGCGCCAAGCTTGGCATCGATGCGCTGGGTTATGAGCCGGAAGAAAAGGAACGGCTG
ATGCATGCCATCGGCCGGCCTTACGGCATGATTTTGGTGACCGGGCCAACGGGTTCAGGGAAAACCGTCTCCTTGTATAC
CTGCCTGAACTTGCTGAACAAGGCAGGCGTCAACATCGCGACCGCGGAGGACCCCTCGGAAATCAATTTGCCTGGTGTCA
ATCAGGTGAACGTCAATGAAAAAGCGGGGCTGACGTTCGCCACCGCACTCAAGTCGTTTCTGCGCCAGGATCCCGACATC
ATCATGGTCGGCGAAATTCGCGATCTTGAAACGGCGGATATTTCCATCAAGGCGGCACAGACGGGCCACTTGGTGCTGTC
CACCCTCCACACCAACGATGCTCCGACCACGCTCACCCGGATGCGCAATATGGGAATTGCGCCATTCAACATTGCGTCCA
GCGTCATTCTGATAACAGCCCAGCGCCTGGCTCGCCGTTTGTGCCCGGTCTGCAAAGCACCCGCCGATATTCCACATGAA
ACCCTGCTCGAGGCAGGCTATAAAGAGGATGAAATCGATGGCACCTGGGTGACCTATCGTCCGGTCGGTTGTTCCGCTTG
CAACAATGGCTACAAGGGGCGGCTGGGCATTTACCAGGTCATGCCGATTTCCGAAGAAATTCAACGCATTATTTTGCGCG
ATGGAAGCGCCTTGGAAATCGCCAAGCAGGCCAAGGCCGAGGGGGTTCGCTCCTTGCGCGAAGCAGGCCTTCATAAAGCC
AAGATGGGGCTGACCTCTCTCGAAGAGGTGCTGGCTGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.767

100

0.545
  pilB Acinetobacter baumannii D1279779

54.059

100

0.543
  pilF Neisseria gonorrhoeae MS11

52.482

97.917

0.514
  pilB Legionella pneumophila strain ERS1305867

49.735

98.09

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

45.552

97.569

0.444
  pilB Vibrio campbellii strain DS40M4

44.938

97.743

0.439
  pilB Vibrio cholerae strain A1552

47.389

89.757

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.895

99.653

0.398
  pilF Thermus thermophilus HB27

38.908

98.611

0.384