Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RZV15_RS16265 Genome accession   NZ_CP136577
Coordinates   3832467..3834203 (-) Length   578 a.a.
NCBI ID   WP_355581793.1    Uniprot ID   -
Organism   Xanthomonas cannabis strain DAR82752     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3827467..3839203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV15_RS16255 (RZV15_16200) - 3828263..3829876 (+) 1614 WP_039405515.1 HAMP domain-containing sensor histidine kinase -
  RZV15_RS16260 (RZV15_16205) pilR 3830401..3831795 (+) 1395 WP_355505398.1 sigma-54 dependent transcriptional regulator Regulator
  RZV15_RS16265 (RZV15_16210) pilB 3832467..3834203 (-) 1737 WP_355581793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RZV15_RS16270 (RZV15_16215) - 3834353..3834955 (-) 603 WP_355581796.1 methionine biosynthesis protein MetW -
  RZV15_RS16275 (RZV15_16220) - 3834948..3835994 (-) 1047 WP_355581798.1 glycosyltransferase family 2 protein -
  RZV15_RS16280 (RZV15_16225) - 3835991..3836785 (-) 795 WP_355581801.1 glycosyltransferase -
  RZV15_RS16285 (RZV15_16230) - 3836789..3837034 (-) 246 WP_355581804.1 SMR family transporter -
  RZV15_RS16290 (RZV15_16235) - 3837514..3837930 (+) 417 WP_039421894.1 pilin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62810.01 Da        Isoelectric Point: 5.6116

>NTDB_id=891655 RZV15_RS16265 WP_355581793.1 3832467..3834203(-) (pilB) [Xanthomonas cannabis strain DAR82752]
MNSVVTANLVGITGIARRLVQDGALEEATARTAMEQAAAAKVPLPQWFAEKKVVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQAGNASFGASLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRKSSLP
ENALLAEGFTPEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=891655 RZV15_RS16265 WP_355581793.1 3832467..3834203(-) (pilB) [Xanthomonas cannabis strain DAR82752]
ATGAATAGCGTCGTCACGGCTAACTTGGTTGGTATCACCGGAATTGCCCGGCGTCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCACTGCGCGGACGGCGATGGAACAGGCGGCTGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAAGGTGG
TGTCGGCGTCTCAGCTGGCGGCAGCCAACGCCGTGGAATTCGGTATGCCGCTGATGGATGTGTCGGTGTTCGACGCCAAT
CAGAACGCGGTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGTATCAAGTGCTGCCACTGTTCAAGCGCGGCAACCGGTT
ATTCGTGGGGGTGAGTAATCCGACCCAGACGCGGGCGTTGGACGACATCAAATTTCACACCAATCTGGTGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGGACGCTGGAGCAGTGGCAGGCAGGTAATGCGTCGTTTGGTGCGTCGCTCGGG
AACGACGATGAGGAGATGGGCGACCTCGACGTCTCTGCCGGAGACGAGGACATGGGCGCCGGCGGGGATTCTGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGCATCGACGGGCTGCTGAAGAACGTGGCCAAAGCGCCG
GTGAAGTTGAATCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATTGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTAAGTACCTTGCCGACCTTGTTTGGCGAGA
AGGTGGTGCTGCGTATCCTGGATGCCAGTGCGGCCAAGCTGGGGATTGAGAAGTTGGGCTACGAGGCGGATCAGCAAAAG
CTGTTCCTGGAAGCGATCCACAAGCCTTATGGCATGGTGCTGGTGACCGGACCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAATGATGAGACGCGCAACATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAACGCGGGATGACGTTTGCTGCGGCGCTGCGCTCGTTCCTGCGCCAAGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACTGGTCACATGGT
GCTGTCCACGTTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGGATTGCGCCGTACAACATCA
CCTCGTCGGTGACCCTGGTCATTGCGCAGCGCCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAGTCGTCGCTGCCT
GAAAATGCGTTGCTTGCTGAAGGTTTTACGCCAGAGCAGATCGCTGCCGGGATCGAACTGTATGAGGCGGTGGGCTGCGA
CGAATGCACCGAAGGCTACAAGGGGCGCACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATTGGCGCCATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATTCGCGATCTGCGGCAGTCGGCGTTGGTG
AAGGCTGCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

98.27

0.557
  pilB Acinetobacter baylyi ADP1

56.162

98.27

0.552
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

50.82

94.983

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilB Vibrio campbellii strain DS40M4

45.694

98.443

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

91.522

0.363
  pilF Thermus thermophilus HB27

40.192

89.965

0.362