Detailed information NTG0089151

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: OCU30_RS11080
• Genome accession: NZ_AP024887
• Coordinates: 2367152..2368477 (-)
• Length: 441 a.a.
• NCBI ID: WP_159439119.1
• Uniprot ID: A0A1R4B478
• Organism: Vibrio palustris strain CECT 9027
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2362152..2373477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCU30_RS11060	-	2362889..2363728 (-)	840	WP_077314116.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
OCU30_RS11065	-	2363799..2365283 (-)	1485	WP_077314118.1	SPOR domain-containing protein	-
OCU30_RS11070	aroB	2365336..2366415 (-)	1080	WP_077314119.1	3-dehydroquinate synthase	-
OCU30_RS11075	aroK	2366446..2366964 (-)	519	WP_077314121.1	shikimate kinase AroK	-
OCU30_RS11080	pilQ	2367152..2368477 (-)	1326	WP_159439119.1	type IV pilus secretin PilQ	Machinery gene
OCU30_RS11085	-	2368467..2368982 (-)	516	WP_077314124.1	hypothetical protein	-
OCU30_RS11090	-	2368975..2369544 (-)	570	WP_077314126.1	hypothetical protein	-
OCU30_RS11095	-	2369531..2370091 (-)	561	WP_159439120.1	PilN domain-containing protein	-
OCU30_RS11100	pilM	2370084..2371034 (-)	951	WP_159439121.1	pilus assembly protein PilM	-

Sequence

Protein

• Length: 441 a.a.
• Molecular weight: 48665.88 Da
• Isoelectric Point: 6.3149

>NTDB_id=89151 OCU30_RS11080 WP_159439119.1 2367152..2368477(-) (pilQ) [Vibrio palustris strain CECT 9027]
MNTRLWSVIMYWCRGGLLLWTMMSFPALAQDEVRDTSSNLISLQFQSIEVRRVLTLLAQKHGFNVVIDEEVTGTISVNLH
NVPWQRALDTILRMKGLSYQADNNVLWVAPLNVIQNYQSKANALAEHRQYQKIPVHYAKADELIEVITAKNGEDVLSTTG
SVQVDKRTNSLLLHDTATHIQRVKELVSMLDVPMKQVLIEARIVTMSQGELVDIGVRWGTFQGQRPTKIAGTLEGLRPTQ
ENSITDDLTVQLPSASPRAASIAFQVATLGPNTLLDLELSALQSESKAEIISSPRLLTTNEKPAYIEQGTEIPYLEAASS
GATSVSFRKAVLSLKVTPHITPNNQMLLDLNVTQDRPGSSVAIGTGDAVAIQTQRIGTQVMVNDGETIVLGGIYQHSLTK
GVEKVPLLGDLPLLGQLFRRSYEEKDKNELVIFVTPKVVIQ

Nucleotide

• Length: 1326 bp

>NTDB_id=89151 OCU30_RS11080 WP_159439119.1 2367152..2368477(-) (pilQ) [Vibrio palustris strain CECT 9027]
ATGAACACTAGATTATGGAGTGTGATTATGTATTGGTGTCGCGGTGGACTCTTGTTATGGACGATGATGTCGTTTCCAGC
TTTGGCTCAGGATGAAGTACGCGATACCTCATCGAATCTCATATCATTACAGTTTCAATCAATCGAAGTGAGAAGAGTCT
TAACACTATTGGCACAAAAGCATGGATTTAATGTGGTGATTGATGAGGAAGTCACCGGAACTATTTCGGTGAATTTACAT
AATGTGCCTTGGCAGCGTGCGCTAGACACCATTTTAAGGATGAAGGGTCTAAGTTATCAAGCGGATAATAATGTGTTGTG
GGTAGCACCGCTCAACGTGATTCAGAATTATCAGAGCAAGGCCAATGCTTTAGCTGAGCATCGGCAATACCAGAAAATTC
CTGTTCATTACGCTAAAGCTGATGAGCTGATTGAAGTAATAACTGCAAAAAATGGAGAGGATGTTTTATCGACCACAGGC
AGTGTACAAGTGGATAAAAGGACAAATTCGTTATTGTTACACGATACCGCGACCCATATACAGCGCGTAAAAGAGCTGGT
GTCTATGCTCGATGTTCCGATGAAACAAGTATTGATTGAAGCGCGTATCGTGACGATGAGCCAAGGCGAGCTAGTTGATA
TTGGTGTTCGGTGGGGAACCTTTCAAGGACAGCGTCCTACGAAGATCGCTGGAACTCTAGAGGGGCTGCGCCCGACGCAG
GAAAACAGTATTACCGATGATCTCACAGTGCAATTACCCAGTGCCTCCCCACGAGCGGCCAGCATTGCGTTTCAGGTGGC
GACATTGGGGCCTAATACCTTGTTAGATCTTGAGCTTTCGGCTTTACAAAGCGAGTCGAAAGCGGAAATTATTTCGAGTC
CGCGTTTACTGACCACCAATGAAAAACCTGCCTATATTGAACAAGGTACTGAAATTCCCTATTTAGAAGCGGCGTCTAGT
GGAGCGACGTCGGTTTCTTTTCGCAAGGCGGTACTCAGTTTGAAGGTGACTCCGCATATTACACCTAACAATCAAATGCT
GTTAGATTTGAATGTAACTCAAGACCGGCCCGGAAGTTCAGTGGCAATTGGTACGGGCGATGCCGTAGCGATTCAAACGC
AGCGAATTGGCACTCAGGTAATGGTCAATGATGGCGAAACGATCGTTTTAGGTGGAATTTATCAACACAGCTTAACGAAA
GGGGTGGAAAAAGTACCACTCTTGGGTGACTTACCGCTGCTCGGACAGCTATTTCGGCGTAGTTATGAAGAAAAAGACAA
AAATGAATTGGTGATATTTGTGACGCCAAAGGTTGTTATTCAATAA

Domains

Predicted by InterproScan.

• Secretin and TonB N terminus short domain (STN) (63-110)

• Bacterial type II/III secretion system short domain (Secretin_N) (133-196)

• Bacterial type II and III secretion system protein (Secretin) (281-439)

3D structure

• AlphaFold DB: A0A1R4B478

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Vibrio campbellii strain DS40M4	55.035	96.825	0.533
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	53.682	95.465	0.512
pilQ	Vibrio cholerae strain A1552	53.682	95.465	0.512
pilQ	Legionella pneumophila strain ERS1305867	42.955	99.773	0.429
pilQ	Legionella pneumophila strain Lp02	42.727	99.773	0.426
comQ	Acinetobacter baylyi ADP1	38.78	100	0.404
comE	Haemophilus influenzae 86-028NP	40.138	98.866	0.397
pilQ	Pseudomonas aeruginosa PAK	36.229	100	0.388
comE	Haemophilus influenzae Rd KW20	43.038	89.569	0.385
comE	Glaesserella parasuis strain SC1401	38.269	99.546	0.381
pilQ	Acinetobacter baumannii D1279779	34.641	100	0.361
pilQ	Acinetobacter baumannii strain A118	34.641	100	0.361