Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RYH83_RS29475 Genome accession   NZ_CP136539
Coordinates   6292625..6294769 (-) Length   714 a.a.
NCBI ID   WP_003161249.1    Uniprot ID   A0A8G2V818
Organism   Pseudomonas aeruginosa strain GOM9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6287625..6299769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RYH83_RS29460 (RYH83_29460) - 6289277..6290932 (-) 1656 WP_003125624.1 AAA family ATPase -
  RYH83_RS29465 (RYH83_29465) aroB 6290939..6292045 (-) 1107 WP_003161251.1 3-dehydroquinate synthase -
  RYH83_RS29470 (RYH83_29470) aroK 6292095..6292613 (-) 519 WP_003161250.1 shikimate kinase AroK -
  RYH83_RS29475 (RYH83_29475) pilQ 6292625..6294769 (-) 2145 WP_003161249.1 type 4a pilus secretin PilQ Machinery gene
  RYH83_RS29480 (RYH83_29480) pilP 6294823..6295347 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  RYH83_RS29485 (RYH83_29485) pilO 6295344..6295967 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  RYH83_RS29490 (RYH83_29490) pilN 6295964..6296560 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  RYH83_RS29495 (RYH83_29495) pilM 6296560..6297624 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77438.99 Da        Isoelectric Point: 5.2285

>NTDB_id=891504 RYH83_RS29475 WP_003161249.1 6292625..6294769(-) (pilQ) [Pseudomonas aeruginosa strain GOM9]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
SGYGKDGNIGIKDEDGMNCGPIAGSCTFPTTGTSKSPSPFVDLGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDYQNMLNGVPPINKN
EVNAKILVNDGETIVIGGVFSNEQSKSVEKVPFLGELPYLGRLFRRDTVTDRKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=891504 RYH83_RS29475 WP_003161249.1 6292625..6294769(-) (pilQ) [Pseudomonas aeruginosa strain GOM9]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGATCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACCGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGTGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGTATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGCGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGTAGCTGTAC
CTTCCCGACCACGGGTACCAGCAAGTCGCCGTCGCCGTTCGTCGACCTCGGTGCCAAGGATGCTACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAATGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTGAAGGGCTCGGAAGTCCCCTACCAGGAGGCCAGTTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAAGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTCAAGGTGACCAAGGACGCGCCGGATTACCAGAATATGTTGAACGGCGTACCTCCTATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGTAACGAGCAGAGCAAGTC
GGTGGAGAAGGTACCCTTCCTCGGCGAACTGCCTTACCTCGGGCGGCTGTTCCGGCGAGATACCGTCACCGACCGGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

100

100

1
  pilQ Acinetobacter baumannii D1279779

40.699

100

0.408
  pilQ Acinetobacter baumannii strain A118

40.699

100

0.408
  comQ Acinetobacter baylyi ADP1

41.168

98.319

0.405
  pilQ Legionella pneumophila strain Lp02

38.102

95.938

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.956

95.938

0.364