Detailed information NTG0890006

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RX793_RS16840
• Genome accession: NZ_CP136263
• Coordinates: 3441665..3442279 (+)
• Length: 204 a.a.
• NCBI ID: WP_041482377.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 0039
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3436665..3447279

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RX793_RS16805 (RX793_16775)	aroE	3437352..3438194 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
RX793_RS16810 (RX793_16780)	yhbY	3438188..3438478 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
RX793_RS16815 (RX793_16785)	-	3438489..3439058 (+)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
RX793_RS16820 (RX793_16790)	yqeK	3439048..3439608 (+)	561	WP_024085627.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RX793_RS16825 (RX793_16795)	rsfS	3439626..3439982 (+)	357	WP_003152864.1	ribosome silencing factor	-
RX793_RS16830 (RX793_16800)	-	3439979..3440716 (+)	738	WP_024085626.1	class I SAM-dependent DNA methyltransferase	-
RX793_RS16835 (RX793_16805)	comER	3440785..3441606 (-)	822	WP_014305449.1	late competence protein ComER	-
RX793_RS16840 (RX793_16810)	comEA	3441665..3442279 (+)	615	WP_041482377.1	helix-hairpin-helix domain-containing protein	Machinery gene
RX793_RS16845 (RX793_16815)	-	3442346..3442915 (+)	570	WP_003152868.1	ComE operon protein 2	-
RX793_RS16850 (RX793_16820)	comEC	3442916..3445267 (+)	2352	WP_024085624.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RX793_RS16855 (RX793_16825)	-	3445286..3445420 (-)	135	WP_003152870.1	YqzM family protein	-
RX793_RS16860 (RX793_16830)	-	3445461..3445615 (+)	155	Protein_3261	hypothetical protein	-
RX793_RS16865 (RX793_16835)	holA	3445655..3446696 (+)	1042	Protein_3262	DNA polymerase III subunit delta	-
RX793_RS16870 (RX793_16840)	rpsT	3446713..3446979 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22222.38 Da
• Isoelectric Point: 6.0523

>NTDB_id=890006 RX793_RS16840 WP_041482377.1 3441665..3442279(+) (comEA) [Bacillus velezensis strain 0039]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=890006 RX793_RS16840 WP_041482377.1 3441665..3442279(+) (comEA) [Bacillus velezensis strain 0039]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCATCCGAAAACAG
CAGCGGGTGAAAAGGAAAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363