Detailed information NTG0088512

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OCUAc17_RS14320
• Genome accession: NZ_AP024798
• Coordinates: 2984169..2985287 (+)
• Length: 372 a.a.
• NCBI ID: WP_016802720.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain OCU Ac17
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2979169..2990287

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCUAc17_RS14310 (OCUAc17_27960)	yggS	2982293..2982985 (-)	693	WP_016802719.1	YggS family pyridoxal phosphate-dependent enzyme	-
OCUAc17_RS14315 (OCUAc17_27970)	pilT	2983112..2984149 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
OCUAc17_RS14320 (OCUAc17_27980)	pilU	2984169..2985287 (+)	1119	WP_016802720.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OCUAc17_RS14325 (OCUAc17_27990)	fur	2985357..2985794 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
OCUAc17_RS14330 (OCUAc17_28000)	omlA	2985906..2986304 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
OCUAc17_RS14335 (OCUAc17_28010)	-	2986369..2986683 (-)	315	WP_002121714.1	RnfH family protein	-
OCUAc17_RS14340 (OCUAc17_28020)	-	2986683..2987750 (-)	1068	WP_005075306.1	hypothetical protein	-
OCUAc17_RS14345 (OCUAc17_28030)	-	2987904..2988359 (-)	456	WP_002121698.1	bacteriohemerythrin	-
OCUAc17_RS14350 (OCUAc17_28040)	-	2988611..2989459 (-)	849	WP_002121748.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41959.20 Da
• Isoelectric Point: 6.6404

>NTDB_id=88512 OCUAc17_RS14320 WP_016802720.1 2984169..2985287(+) (pilU) [Acinetobacter pittii strain OCU Ac17]
MDFNDLLNLMVEKKSSDLFITDGLAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=88512 OCUAc17_RS14320 WP_016802720.1 2984169..2985287(+) (pilU) [Acinetobacter pittii strain OCU Ac17]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCCTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGTGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.733	99.731	0.685
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.209	97.849	0.403
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363