Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RKE25_RS17975 Genome accession   NZ_CP134867
Coordinates   4070481..4072202 (+) Length   573 a.a.
NCBI ID   WP_311839458.1    Uniprot ID   A0AA96IZF2
Organism   Dyella sp. BiH032     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4065481..4077202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE25_RS17955 (RKE25_17955) - 4065790..4066836 (+) 1047 WP_311839454.1 glycosyltransferase -
  RKE25_RS17960 (RKE25_17960) - 4066868..4067818 (-) 951 WP_311839455.1 glycosyltransferase family 2 protein -
  RKE25_RS17965 (RKE25_17965) - 4067920..4069368 (-) 1449 WP_311839456.1 oligosaccharide flippase family protein -
  RKE25_RS17970 (RKE25_17970) - 4069365..4070243 (-) 879 WP_311839457.1 glycosyltransferase family 2 protein -
  RKE25_RS17975 (RKE25_17975) pilB 4070481..4072202 (+) 1722 WP_311839458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RKE25_RS17980 (RKE25_17980) pilC 4072343..4073608 (+) 1266 WP_311839459.1 type II secretion system F family protein Machinery gene
  RKE25_RS17985 (RKE25_17985) pilD 4073683..4074543 (+) 861 WP_311839460.1 A24 family peptidase Machinery gene
  RKE25_RS17990 (RKE25_17990) coaE 4074540..4075160 (+) 621 WP_311839461.1 dephospho-CoA kinase -
  RKE25_RS17995 (RKE25_17995) - 4075169..4076113 (-) 945 WP_311839462.1 Nudix family hydrolase -
  RKE25_RS18000 (RKE25_18000) - 4076134..4076622 (-) 489 WP_311839463.1 dihydrofolate reductase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62669.28 Da        Isoelectric Point: 6.5117

>NTDB_id=884210 RKE25_RS17975 WP_311839458.1 4070481..4072202(+) (pilB) [Dyella sp. BiH032]
MATQMQPSLSGLSGMARRLVSEGVLPEPDVRKAVADSAQHKTSLTSWLLDNNLVESARLTQIASAEFGMPAMDVSSLQPT
SMPLNLISEALVNKHHALPLFRRGKRLFVGIADPMQSHALDEIKFHSNCMVEPILVERGQLHRVIESALNTMNATVPDLG
GDDLEELALEAGDDDGDTTGIDATANEDAPVVKFVNKILVDAIKRGASDIHFEPFETQYRVRLRMDGMLRAVASPPMKLA
TRISSRLKVMAGLDIAERRVPQDGRIKLNLSKSRSVDFRVSTLPTLFGEKIVLRILDASTAKLGIDKLGYEEVQKQLYLD
AIHKPYGMVLVTGPTGSGKTVSLYTALNILNTNERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLHDCKKPMDLPPQTLL
AAGFTQEEIDAGLTLFEAVGCDHCNEGYKGRVGIYQVMPMLEDIQKIVLQGGNAMQIAEVARKAGINDLRASALMKVKQG
VTSLAEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=884210 RKE25_RS17975 WP_311839458.1 4070481..4072202(+) (pilB) [Dyella sp. BiH032]
ATGGCGACGCAAATGCAACCGTCTTTGTCGGGCCTGTCAGGCATGGCCCGGCGACTGGTGTCCGAGGGGGTGCTCCCCGA
GCCGGACGTGCGCAAGGCGGTGGCCGACTCGGCCCAGCACAAGACCTCGCTCACCTCCTGGCTGCTCGACAACAACCTGG
TCGAAAGCGCCCGCCTGACCCAGATCGCCTCCGCCGAGTTCGGCATGCCGGCCATGGATGTCTCGTCGCTGCAGCCGACC
AGCATGCCGCTCAACCTGATCAGCGAGGCGCTGGTCAACAAGCACCATGCCCTGCCCCTGTTCCGGCGCGGCAAGCGCCT
GTTCGTCGGCATCGCCGACCCCATGCAGTCCCACGCGCTGGACGAGATCAAGTTCCACTCCAACTGCATGGTGGAACCGA
TCCTGGTCGAGCGCGGGCAGCTGCACCGGGTGATCGAGAGCGCGCTCAACACGATGAATGCCACCGTCCCGGATCTCGGC
GGGGACGATCTGGAAGAACTGGCGCTGGAAGCCGGCGACGACGACGGCGACACCACGGGCATCGACGCCACCGCGAACGA
AGACGCGCCGGTCGTCAAGTTCGTCAACAAGATCCTGGTCGATGCGATCAAGCGCGGCGCGTCGGACATTCATTTCGAGC
CATTCGAAACCCAGTACCGGGTGCGCCTGCGCATGGACGGCATGCTGCGCGCGGTGGCCAGCCCGCCGATGAAACTGGCG
ACGCGTATTTCCTCGCGCCTGAAGGTGATGGCTGGCCTGGACATCGCCGAGCGCCGCGTCCCGCAGGACGGCCGTATCAA
GCTCAACCTCTCCAAGAGCCGCTCGGTCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGATCGTGCTGC
GTATCCTGGACGCCTCCACGGCGAAGCTGGGCATCGACAAGCTGGGTTACGAGGAGGTGCAGAAGCAGCTCTACCTCGAC
GCCATCCACAAGCCCTACGGCATGGTGCTCGTCACCGGCCCCACGGGTTCGGGTAAGACGGTGTCGCTGTACACCGCGCT
CAATATCCTCAATACCAACGAGCGCAATATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGGAAGGCATCAACCAGG
TCCAGCAGAACACCAAGCGCGGCATGACCTTCGCGGCGGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGACGTGATCATG
GTCGGCGAAATCCGCGACCTGGAAACGGCTGAGATCGCCATCAAGGCCGCGCAGACCGGTCACATGGTGTTGTCCACCCT
GCATACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCTTCGGTCA
CCCTGGTGATCGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGAAGCCGATGGACCTGCCGCCGCAGACGCTGCTG
GCCGCAGGCTTTACACAGGAAGAGATCGACGCCGGCCTGACCCTTTTCGAAGCCGTGGGCTGCGACCACTGCAACGAGGG
CTACAAGGGCCGCGTGGGCATCTATCAGGTGATGCCGATGCTGGAGGACATCCAGAAGATCGTGCTGCAGGGCGGCAACG
CGATGCAGATCGCCGAGGTGGCCCGCAAGGCCGGCATCAACGACCTGCGCGCATCGGCCCTCATGAAGGTCAAGCAGGGC
GTGACCAGCCTCGCCGAAATCGATCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.196

99.825

0.541
  pilB Acinetobacter baumannii D1279779

54.497

98.953

0.539
  pilB Legionella pneumophila strain ERS1305867

50.788

99.651

0.506
  pilF Neisseria gonorrhoeae MS11

47.518

98.429

0.468
  pilB Vibrio cholerae strain A1552

48.519

94.241

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

48.972

93.368

0.457
  pilB Vibrio campbellii strain DS40M4

48.224

93.368

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.194

95.288

0.373
  pilF Thermus thermophilus HB27

36.649

100

0.366