Detailed information NTG0883612

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RNI18_RS18490
• Genome accession: NZ_CP134775
• Coordinates: 3667006..3667620 (+)
• Length: 204 a.a.
• NCBI ID: WP_003152867.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain KB21
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3662006..3672620

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RNI18_RS18455 (RNI18_18455)	aroE	3662693..3663535 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
RNI18_RS18460 (RNI18_18460)	yhbY	3663529..3663819 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
RNI18_RS18465 (RNI18_18465)	-	3663830..3664399 (+)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
RNI18_RS18470 (RNI18_18470)	yqeK	3664389..3664949 (+)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RNI18_RS18475 (RNI18_18475)	rsfS	3664967..3665323 (+)	357	WP_003152864.1	ribosome silencing factor	-
RNI18_RS18480 (RNI18_18480)	-	3665320..3666057 (+)	738	WP_032856885.1	class I SAM-dependent methyltransferase	-
RNI18_RS18485 (RNI18_18485)	comER	3666126..3666947 (-)	822	WP_014305449.1	late competence protein ComER	-
RNI18_RS18490 (RNI18_18490)	comEA	3667006..3667620 (+)	615	WP_003152867.1	helix-hairpin-helix domain-containing protein	Machinery gene
RNI18_RS18495 (RNI18_18495)	-	3667687..3668256 (+)	570	WP_003152868.1	ComE operon protein 2	-
RNI18_RS18500 (RNI18_18500)	comEC	3668257..3670608 (+)	2352	WP_041481887.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RNI18_RS18505 (RNI18_18505)	-	3670627..3670761 (-)	135	WP_003152870.1	YqzM family protein	-
RNI18_RS18510 (RNI18_18510)	-	3670802..3670956 (+)	155	Protein_3592	hypothetical protein	-
RNI18_RS18515 (RNI18_18515)	holA	3670996..3672037 (+)	1042	Protein_3593	DNA polymerase III subunit delta	-
RNI18_RS18520 (RNI18_18520)	rpsT	3672054..3672320 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22269.48 Da
• Isoelectric Point: 6.4940

>NTDB_id=883612 RNI18_RS18490 WP_003152867.1 3667006..3667620(+) (comEA) [Bacillus velezensis strain KB21]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=883612 RNI18_RS18490 WP_003152867.1 3667006..3667620(+) (comEA) [Bacillus velezensis strain KB21]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	59.375	94.118	0.559
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363