Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RMV19_RS27565 Genome accession   NZ_CP134773
Coordinates   6132630..6134708 (-) Length   692 a.a.
NCBI ID   WP_311885152.1    Uniprot ID   -
Organism   Pseudomonas sp. VD-NE white     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6127630..6139708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RMV19_RS27550 - 6129251..6130843 (-) 1593 WP_034152953.1 AAA family ATPase -
  RMV19_RS27555 aroB 6130854..6131954 (-) 1101 WP_034152954.1 3-dehydroquinate synthase -
  RMV19_RS27560 aroK 6132107..6132625 (-) 519 WP_025110261.1 shikimate kinase AroK -
  RMV19_RS27565 pilQ 6132630..6134708 (-) 2079 WP_311885152.1 type IV pilus secretin PilQ Machinery gene
  RMV19_RS27570 - 6134722..6135249 (-) 528 WP_034152956.1 pilus assembly protein PilP -
  RMV19_RS27575 pilO 6135246..6135869 (-) 624 WP_016983853.1 type 4a pilus biogenesis protein PilO -
  RMV19_RS27580 - 6135866..6136432 (-) 567 WP_034152958.1 PilN domain-containing protein -
  RMV19_RS27585 pilM 6136432..6137496 (-) 1065 WP_311885147.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75155.75 Da        Isoelectric Point: 5.8322

>NTDB_id=883518 RMV19_RS27565 WP_311885152.1 6132630..6134708(-) (pilQ) [Pseudomonas sp. VD-NE white]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLPNKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYSTRVEGNNLFVVVGQGAPAPVSRPAAVAPRVTAKAPAPARAFVPKTRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAGVSADRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=883518 RMV19_RS27565 WP_311885152.1 6132630..6134708(-) (pilQ) [Pseudomonas sp. VD-NE white]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGACGTGGCGGCATTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCACAACCCAAGG
GCTACACCACCGAATCGCCGGCACGGATTGCGCTGGACCTGCCCGGTGTCGCCAGTCAGTTGCCCAACAAGAACCTCGAT
CTGGGTAGTGGCAATGCGCGCACGGCCACGGTGGTTGAGGCCAAGGAGCGGACGCGGCTGATTGTCAGCCTGACGCAGCT
CGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTCGTAGTGGTCGGTCAGGGCGCACCGGCACCCGTGTCGCGTC
CTGCTGCCGTTGCGCCGCGCGTTACCGCTAAAGCGCCTGCGCCCGCCAGGGCATTTGTGCCGAAAACCCGGGCGATTCGC
GGTGTGGACTTCCAGCGCGGTACGGCAGGTGAAGGCAATGTCGTCATCGACCTGTCTGACCCGACCATCGCTCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCGGAAAAGCTGCGCGTACGCCTCGACGTCA
AGGATTTCGCCACACCGGTGCAATTCGTCAATGCCGGAGTGAGCGCTGATCGCACGGTTATTACCGTTGAACCCAGTGGC
ACCTACGAGTACTCCACCTTCCAGACCGACAACAAACTGACCGTCAGCATCCGGCCGATGACCGTCGATGATCTGCAAAA
GCGTAACGCAGATCGTCAGGCTTACGTCGGCGAAAAGCTCTCGCTGAATTTTCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTGGTCGCCAGCGATACGGTGCAGGGTGGCATCACTTTGCGTCTGCAAAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAAGGCCTGGATAAACGCAAGATCGGCAATGTGCTGCTGGT
GGCGCCGGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAGCTGTTGCAGGTGAACTACGCCAAAGCCGCGGACATTGCCAAGCTGTTTCAGTCGGTGACCAGTGCCGAGGCG
AAAATCGACGAACGCGGTTCGATTACAGTGGATGAGCGGACCAATAACATCATTGCCTATCAGACGCAGGATCGACTCGA
TGAGCTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCGCGAATTGTCGAAGCCAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACGCTTCCGGGGTCAAC
GGTTCATCGACGACCATCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACAGTGGGCAATAC
CTCAGGGATCGGCATCGCTTTCATCACCGACAACGTGCTGCTCGATCTAGAGCTGACCGCGATGGAGAAGACCGGTAACG
GCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGTTCCAGTGGCGCGACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
CCCCGACAACCGCATCATCATGGAGGTCAAAGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.587

100

0.75
  comQ Acinetobacter baylyi ADP1

41.354

100

0.415
  pilQ Acinetobacter baumannii strain A118

38.327

100

0.41
  pilQ Acinetobacter baumannii D1279779

38.327

100

0.41
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.688

0.371
  pilQ Legionella pneumophila strain Lp02

38.018

97.688

0.371