Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHP75_RS17490 Genome accession   NZ_CP134499
Coordinates   3676455..3678158 (+) Length   567 a.a.
NCBI ID   WP_311089310.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20056     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3671455..3683158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP75_RS17475 (RHP75_17475) nadC 3672910..3673758 (+) 849 WP_311089307.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHP75_RS17480 (RHP75_17480) - 3673895..3675589 (-) 1695 WP_311089308.1 Wzy polymerase domain-containing protein -
  RHP75_RS17485 (RHP75_17485) - 3675815..3676234 (-) 420 WP_311089309.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RHP75_RS17490 (RHP75_17490) pilB 3676455..3678158 (+) 1704 WP_311089310.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHP75_RS17495 (RHP75_17495) pilC 3678162..3679379 (+) 1218 WP_311089311.1 type II secretion system F family protein Machinery gene
  RHP75_RS17500 (RHP75_17500) pilD 3679383..3680252 (+) 870 WP_311089312.1 A24 family peptidase Machinery gene
  RHP75_RS17505 (RHP75_17505) coaE 3680407..3681021 (+) 615 WP_311089313.1 dephospho-CoA kinase -
  RHP75_RS17510 (RHP75_17510) yacG 3681018..3681218 (+) 201 WP_305589768.1 DNA gyrase inhibitor YacG -
  RHP75_RS17515 (RHP75_17515) - 3681222..3681437 (-) 216 WP_311089314.1 hypothetical protein -
  RHP75_RS17520 (RHP75_17520) - 3681542..3682150 (-) 609 WP_311089315.1 energy-coupling factor ABC transporter permease -
  RHP75_RS17525 (RHP75_17525) - 3682283..3682912 (+) 630 WP_160013159.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62490.48 Da        Isoelectric Point: 5.4733

>NTDB_id=880576 RHP75_RS17490 WP_311089310.1 3676455..3678158(+) (pilB) [Pseudomonas sp. SG20056]
MNESVSLTGLARQMVLAELLDEKAAQQAQSQAQRNKLSLVTYLVQNKLVKSRVLAELASEQFGVSFVDLNAIDKESQPKD
LVTEKLVRQHRVLPLWRRGNKLFIAVSDPTNHQAVTDIQFSTGMNTEAILVEDDKLGEAIEKFFDSAASGMEDLGDIDLD
GLDVEAVNDDTQDKGEGNAADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEIARPPIQLSPRISAR
LKVMAGLDISERRKPQDGRIKMRISKSKSIDFRVSTCPTLWGEKIVMRILDSSSAQMGIDALGYEESQKDLYMSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEIQVPRETLLEEGFP
ENKIGTFKIYEPVGCESCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDISIQMRKDGFNDLRTSALLKAMQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=880576 RHP75_RS17490 WP_311089310.1 3676455..3678158(+) (pilB) [Pseudomonas sp. SG20056]
ATGAACGAAAGCGTTTCCCTTACGGGCCTGGCACGGCAAATGGTGCTGGCCGAGCTGCTGGACGAAAAAGCAGCCCAGCA
GGCACAAAGCCAAGCCCAGCGAAATAAATTGTCGCTTGTCACATACTTGGTGCAGAACAAGCTGGTCAAGAGCCGCGTTC
TGGCCGAGCTTGCATCTGAACAATTCGGTGTGTCTTTTGTTGATTTGAATGCAATAGACAAGGAAAGCCAACCCAAAGAC
TTGGTGACCGAAAAACTGGTACGCCAGCACCGAGTGCTGCCGCTATGGCGGCGCGGTAATAAGCTTTTTATTGCCGTGTC
CGACCCCACCAACCATCAAGCGGTCACTGATATACAGTTCAGCACTGGGATGAATACAGAAGCTATCCTGGTAGAAGACG
ACAAGCTTGGTGAAGCAATCGAGAAATTCTTCGACTCAGCCGCAAGCGGCATGGAGGATCTCGGCGATATTGATCTTGAT
GGCCTTGACGTCGAAGCCGTCAACGATGACACGCAAGATAAAGGGGAAGGTAATGCGGCCGATGACGCACCGGTGGTGCG
CTTTGTTAACAAGATGCTGCTGGATGCCATTAAAGGCGGCTCTTCGGATCTGCATTTTGAGCCTTACGAGAAAGCCTACC
GTGTGCGCTTCCGCACCGACGGCATTCTGCACGAGATAGCTCGTCCGCCCATCCAGTTATCACCACGCATTTCTGCTCGA
CTTAAGGTTATGGCAGGCCTGGATATTTCCGAACGACGCAAACCGCAGGATGGGCGCATCAAGATGCGCATCTCAAAAAG
CAAGTCTATCGACTTCCGCGTCAGTACTTGCCCTACGCTATGGGGTGAGAAGATCGTAATGCGAATTCTTGACTCTTCAA
GCGCCCAGATGGGAATCGACGCCCTTGGCTATGAAGAATCGCAAAAAGATTTATATATGTCCGCCCTGAAGCAGCCTCAG
GGCATGATTTTGGTAACGGGCCCTACCGGCTCGGGCAAGACTGTATCTCTCTACACTGGCTTGAACATTCTCAATACCGT
CGACGTAAATATCTCAACCGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCAAAC
AAGGCATGGACTTTACCGCAGCACTTAAATCGTTCCTGCGCCAGGATCCTGACATCATCATGGTTGGTGAGATACGCGAC
CTAGATACCGCATCTATCGCCATCAAAGCAGCACAAACCGGTCACATGGTTATGTCAACCCTACACACCAATAGCGCAGC
AGAAACACTGACTCGCCTGCGCAATATGGGCGTTCCCTCATTTAACATTGCCACCTCGGTTAACCTGATCATCGCCCAGC
GCCTAGCTCGTAAGCTCTGCAATAGCTGTAAGAAGGAAATACAGGTCCCTCGGGAAACCTTGCTTGAGGAGGGGTTCCCG
GAAAATAAAATCGGCACATTTAAGATTTATGAGCCCGTAGGATGCGAAAGCTGCAATGGTGGCTACAAAGGTCGTGTCGG
AATTTATGAAGTGGTTAAAAACACGCCTGCTCTACAACGGATTATCATGGAGGAAGGCAACTCTATTGATATTTCCATAC
AGATGCGCAAAGACGGCTTTAACGACCTGCGTACCTCAGCACTGCTGAAGGCTATGCAAGGCGTGACCAGCCTCGCAGAA
GTGAACCGCGTGACTAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.596

100

0.589
  pilB Acinetobacter baylyi ADP1

57.904

99.295

0.575
  pilB Legionella pneumophila strain ERS1305867

55.517

100

0.559
  pilB Vibrio cholerae strain A1552

50.709

99.471

0.504
  pilB Vibrio campbellii strain DS40M4

49.291

99.471

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.201

95.414

0.489
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

93.651

0.365
  pilF Thermus thermophilus HB27

36.252

100

0.365